CDK9 Polyclonal antibody
CDK9 Polyclonal Antibody for FC, IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, IF, FC
Conjugate
Unconjugated
Cat no : 11705-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, A431 cells, human placenta tissue, HEK-293 cells, Jurka cells |
Positive IHC detected in | human gliomas tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 4 publications below |
IHC | See 1 publications below |
IP | See 1 publications below |
Product Information
11705-1-AP targets CDK9 in WB, IP, IHC, IF, FC applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CDK9 fusion protein Ag2318 |
Full Name | cyclin-dependent kinase 9 |
Calculated molecular weight | 372 aa, 43 kDa |
Observed molecular weight | 43 kDa, 55 kDa |
GenBank accession number | BC001968 |
Gene symbol | CDK9 |
Gene ID (NCBI) | 1025 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).
Protocols
Product Specific Protocols | |
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WB protocol for CDK9 antibody 11705-1-AP | Download protocol |
IHC protocol for CDK9 antibody 11705-1-AP | Download protocol |
IF protocol for CDK9 antibody 11705-1-AP | Download protocol |
FC protocol for CDK9 antibody 11705-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis CSN6 promotes melanoma proliferation and metastasis by controlling the UBR5-mediated ubiquitination and degradation of CDK9. | ||
Chem Biol Drug Des Synthesis, Structure-Activity Relationship Studies and Biological Evaluation of Novel 2,5-Disubstituted Indole Derivatives as Anticancer Agents. | ||
Proteomics A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand. | ||
Nat Commun The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression. |