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CDK9 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 11705-1-AP

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Con: 60 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

CDK9 Antibody WB A431 cells 11705-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CDK9 Antibody WB Jurkat cells 11705-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 11705-1-AP( CDK9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CDK9 Antibody IHC human gliomas tissue 11705-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CDK9 Antibody IHC human gliomas tissue 11705-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, A431 cells, human placenta tissue, HEK-293 cells, Jurka cells
Positive IHC detected in:
human gliomas tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CDK9 fusion protein Ag2318
Full name:
cyclin-dependent kinase 9
Calculated molecular weight:
372aa,43 kDa
Observed molecular weight:
43 kDa, 55 kDa
GenBank accession number:
BC001968
Gene ID (NCBI):
1025
Gene symbol
CDK9
Synonyms
C 2k, CDC2L4, CDK9, Cell division protein kinase 9, CTK1, cyclin dependent kinase 9, PITALRE, TAK

Background

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).


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