CDK9 Polyclonal antibody

CDK9 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11705-1-AP

Synonyms

C 2k, CDC2L4, CDK9, Cell division protein kinase 9, CTK1, cyclin dependent kinase 9, PITALRE, TAK



Tested Applications

Positive WB detected inJurkat cells, A431 cells, human placenta tissue, HEK-293 cells, Jurka cells
Positive IHC detected inhuman gliomas tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11705-1-AP targets CDK9 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK9 fusion protein Ag2318
Full Name cyclin-dependent kinase 9
Calculated molecular weight 372 aa, 43 kDa
Observed molecular weight 43 kDa, 55 kDa
GenBank accession numberBC001968
Gene symbol CDK9
Gene ID (NCBI) 1025
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).

Protocols

Product Specific Protocols
WB protocol for CDK9 antibody 11705-1-APDownload protocol
IHC protocol for CDK9 antibody 11705-1-APDownload protocol
IF protocol for CDK9 antibody 11705-1-APDownload protocol
FC protocol for CDK9 antibody 11705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

CSN6 promotes melanoma proliferation and metastasis by controlling the UBR5-mediated ubiquitination and degradation of CDK9.

Authors - Yanli Zhang

Chem Biol Drug Des

Synthesis, Structure-Activity Relationship Studies and Biological Evaluation of Novel 2,5-Disubstituted Indole Derivatives as Anticancer Agents.

Authors - Hongyu Hu
humanWB

Proteomics

A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand.

Authors - Xiaolei Zhang
humanWB

Nat Commun

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Authors - Gongwei Wu