CDK9 Polyclonal antibody

CDK9 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC

Conjugate

Unconjugated

Publications

5

Cat no : 11705-1-AP

Synonyms

C 2k, CDC2L4, CDK9, Cell division protein kinase 9, CTK1, cyclin dependent kinase 9, PITALRE, TAK

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Validation Data Gallery

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  WB analysis of Jurkat using 11705-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11705-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11705-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11705-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11705-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11705-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11705-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11705-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (11705-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11705-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CDK9 (11705-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Tested Applications

• Positive WB detected in: Jurkat cells, A431 cells, human placenta tissue, HEK-293 cells, Jurka cells
• Positive IHC detected in: human gliomas tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Planning an IF experiment? We recommend our CoraLite®488, CoraLite®555 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

11705-1-AP targets CDK9 in WB, IP, IHC, IF, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK9 fusion protein Ag2318
• Full Name: cyclin-dependent kinase 9
• Calculated molecular weight: 372 aa, 43 kDa
• Observed molecular weight: 43 kDa, 55 kDa
• GenBank accession number: BC001968
• Gene symbol: CDK9
• Gene ID (NCBI): 1025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis; CSN6 promotes melanoma proliferation and metastasis by controlling the UBR5-mediated ubiquitination and degradation of CDK9.; Authors - Yanli Zhang
		Chem Biol Drug Des; Synthesis, Structure-Activity Relationship Studies and Biological Evaluation of Novel 2,5-Disubstituted Indole Derivatives as Anticancer Agents.; Authors - Hongyu Hu
human	WB	Proteomics; A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand.; Authors - Xiaolei Zhang
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human,mouse	WB,IP	Nat Commun; The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.; Authors - Xinzhi Li