|Positive WB detected in||Jurkat cells, A431 cells, human placenta tissue, HEK-293 cells, Jurka cells|
|Positive IHC detected in||human gliomas tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11705-1-AP targets CDK9 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CDK9 fusion protein Ag2318|
|Full Name||cyclin-dependent kinase 9|
|Calculated molecular weight||372 aa, 43 kDa|
|Observed molecular weight||43 kDa, 55 kDa|
|GenBank accession number||BC001968|
|Gene ID (NCBI)||1025|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).
|Product Specific Protocols|
|WB protocol for CDK9 antibody 11705-1-AP||Download protocol|
|IHC protocol for CDK9 antibody 11705-1-AP||Download protocol|
|IF protocol for CDK9 antibody 11705-1-AP||Download protocol|
|FC protocol for CDK9 antibody 11705-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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