Multi-rAb® CDKN2A/P16-INK4A Multi-Recombinant antibody

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• Multi-rAb® CDKN2A/P16-INK4A Multi-Recombinant antibody (RMX00038)

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  WB analysis using RMX00038

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00038 (CDKN2A/P16-INK4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using RMX00038

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00038 (CDKN2A/P16-INK4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using RMX00038

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using RMX00038 (CDKN2A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using RMX00038

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDKN2A antibody (RMX00038) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CDKN2A/P16-INK4A Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00038

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CDKN2A, P16 INK4A, P16-INK4A, CDK4I, CDKN2

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, HeLa cells, TF-1 cells, C6 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00038 targets CDKN2A/P16-INK4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: cyclin-dependent kinase inhibitor 2A
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: BC021998
• Gene Symbol: CDKN2A
• Gene ID (NCBI): 1029
• RRID: AB_3741448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P42771
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively-spliced variants encoding distinct proteins proteins were reported. Two of them named p16-INK4 and p14 are sharing 50% identity. The third one -p14(ARF) is entirely unrelated. P16 plays an essential role in regulating the cell cycle, and mutations in p16 increase the risk of developing various cancers, including melanoma.