CDS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDS2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13175-1-AP

Synonyms

CDP DAG synthase 2, CDP DG synthase 2, CDP diacylglycerol synthase 2, CDP diglyceride synthase 2, CDS 2, CDS2

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Validation Data Gallery

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  WB analysis of mouse brain using 13175-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13175-1-AP (CDS2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13175-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13175-1-AP (CDS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13175-1-AP

  IP result of anti-CDS2 (IP:13175-1-AP, 4ug; Detection:13175-1-AP 1:300) with HepG2 cells lysate 1000ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

13175-1-AP targets CDS2 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDS2 fusion protein Ag3842
• Full Name: CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2
• Calculated Molecular Weight: 445 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC025751
• Gene Symbol: CDS2
• Gene ID (NCBI): 8760
• RRID: AB_2291458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; AGPAT2 interaction with CDP-diacylglycerol synthases promotes the flux of fatty acids through the CDP-diacylglycerol pathway.; Authors - Hoi Yin Mak ; KD Validated
zebrafish	IF	Nat Commun; Anti-angiogenic effects of VEGF stimulation on endothelium deficient in phosphoinositide recycling.; Authors - Amber N Stratman
human	WB	Sci Rep; Enzymatic fluorometric assays for quantifying all major phospholipid classes in cells and intracellular organelles.; Authors - Tokuji Tsuji