CDSN Polyclonal antibody

CDSN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27953-1-AP

Synonyms

S protein, HTSS, D6S586E, corneodesmosin

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Validation Data Gallery

• 

  WB analysis of mouse skin using 27953-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 27953-1-AP (CDSN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brown disease using 27953-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 27953-1-AP (CDSN antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skin tissue
• Positive IHC detected in: human brown disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

27953-1-AP targets CDSN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDSN fusion protein Ag27688
• Full Name: corneodesmosin
• Calculated Molecular Weight: 528 aa, 52 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC031993
• Gene Symbol: CDSN
• Gene ID (NCBI): 1041
• RRID: AB_2881022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	JID Innov; Loricrin and NRF2 Coordinate Cornification.; Authors - Yosuke Ishitsuka