Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 21655-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 21655-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
mouse colon tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 21655-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 21655-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse stomach using 21655-1-AP
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 21655-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of Caco-2 using 21655-1-AP
IP result of anti-CDX1 (IP:21655-1-AP, 4ug; Detection:21655-1-AP 1:1000) with Caco-2 cells lysate 1400 ug.
IP result of anti-CDX1 (IP:21655-1-AP, 4ug; Detection:21655-1-AP 1:1000) with Caco-2 cells lysate 1400 ug.
IHC staining of human colon cancer using 21655-1-AP
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21655-1-AP (CDX1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21655-1-AP (CDX1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Caco-2 using 21655-1-AP
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX1 antibody (21655-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX1 antibody (21655-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Caco-2 cells, mouse small intestine tissue, mouse stomach tissue, mouse colon tissue, rat colon tissue
Positive IP detected in
Caco-2 cells
Positive IHC detected in
human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Caco-2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
21655-1-AP targets CDX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 21655-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 21655-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 21655-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 21655-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse stomach using 21655-1-AP
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 21655-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon cancer using 21655-1-AP
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21655-1-AP (CDX1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of Caco-2 using 21655-1-AP
IP result of anti-CDX1 (IP:21655-1-AP, 4ug; Detection:21655-1-AP 1:1000) with Caco-2 cells lysate 1400 ug.
IF/ICC Figures
IF Staining of Caco-2 using 21655-1-AP
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX1 antibody (21655-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CDX1 Polyclonal antibody
Catalog Number
21655-1-AP
Citations
-
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
