CDX1 Polyclonal antibody

CDX1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 21655-1-AP

Synonyms

caudal type homeobox 1, Caudal type homeobox protein 1, CDX1, Homeobox protein CDX 1

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Validation Data Gallery

• 

  WB analysis of mouse colon using 21655-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21655-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21655-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 21655-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21655-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 21655-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 21655-1-AP (CDX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse small intestine tissue, mouse stomach tissue
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21655-1-AP is CDX1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDX1 fusion protein Ag16376
• Full Name: caudal type homeobox 1
• Calculated molecular weight: 265 aa, 28 kDa
• Observed molecular weight: 28 kDa
• GenBank accession number: BC096252
• Gene symbol: CDX1
• Gene ID (NCBI): 1044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.