CEACAM-1/CD66a Polyclonal antibody

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  WB analysis using 32395-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 32395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 32395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 32395-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CEACAM-1/CD66a antibody (32395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CEACAM-1/CD66a Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 32395-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF-P, ELISA

Synonyms

Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein D, Biliary glycoprotein 1, BGP-1, Bgp1

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse liver tissue, mouse small intestine
• Positive IF-P detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32395-1-AP targets CEACAM-1/CD66a in WB, IF-P, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: carcinoembryonic antigen-related cell adhesion molecule 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 100-120 kDa
• Gene Symbol: Ceacam1
• Gene ID (NCBI): 26365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P31809-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).