CEBPG Polyclonal antibody

• Featured Product
• KD/KO Validated

CEBPG Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12997-1-AP

Synonyms

C/EBP gamma, CEBPG, GPE1BP, IG/EBP 1

---

Validation Data Gallery

• 

  IHC staining of mouse liver using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 12997-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CEBPG antibody (12997-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Tested Applications

• Positive IHC detected in: mouse liver tissue, mouse colon tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

12997-1-AP targets CEBPG in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEBPG fusion protein Ag3663
• Full Name: CCAAT/enhancer binding protein (C/EBP), gamma
• Calculated Molecular Weight: 150 aa, 16 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: BC013128
• Gene Symbol: CEBPG
• Gene ID (NCBI): 1054
• RRID: AB_2877902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	Neuron; Core transcription programs controlling injury-induced neurodegeneration of retinal ganglion cells.; Authors - Feng Tian ; KD Validated
human	WB,IHC	Am J Cancer Res; CEBPG promotes esophageal squamous cell carcinoma progression by enhancing PI3K-AKT signaling.; Authors - Yongsheng Huang ; KD Validated
human,mouse	WB,IHC	J Transl Med; CEBPG suppresses ferroptosis through transcriptional control of SLC7A11 in ovarian cancer; Authors - Xiaoqian Zhang