CELA2A Polyclonal antibody

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  WB analysis using 33375-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33375-1-AP (CELA2A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

CELA2A Polyclonal Antibody for WB, ELISA

Cat No. 33375-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Chymotrypsin-like elastase family member 2A, Ela2, Ela-2, Ela2a, Elastase-2

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33375-1-AP targets CELA2A in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg3064

  Product name: recombinant mouse CELA2A protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 17-271 aa of NM_007919.3

  Sequence: CGYPTYEVEDDVSRVVGGQEATPNTWPWQVSLQVLSSGRWRHNCGGSLVANNWVLTAAHCLSNYQTYRVLLGAHSLSNPGAGSAAVQVSKLVVHQRWNSQNVGNGYDIALIKLASPVTLSKNIQTACLPPAGTILPRNYVCYVTGWGLLQTNGNSPDTLRQGRLLVVDYATCSSASWWGSSVKSSMVCAGGDGVTSSCNGDSGGPLNCRASNGQWQVHGIVSFGSSLGCNYPRKPSVFTRVSNYIDWINSVMARN

• Full Name: elastase 2A
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: NM_007919.3
• Gene Symbol: RP23-395H4.4
• Gene ID (NCBI): 13706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P05208
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CELA2A (Chymotrypsin-like elastase family member 2A), also known as Elastase-2A, is a circulating enzyme that reduces platelet hyperactivation, triggers both insulin secretion and degradation, and increases insulin sensitivity. ELA-2 is widely found in several organs such as lung, pancreas, liver, blood vessels (mesenteric, and carotid arteries), heart and kidney of rodents (PMID: 31358993, 28386233).