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  • KD/KO Validated

CENPF Polyclonal antibody

CENPF Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat and More (1)





Cat no : 28568-1-AP


AH antigen, CENF, CENP F, CENPF, Centromere protein F, hcp 1, Kinetochore protein CENPF, Mitosin, PRO1779

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inrat small intestine tissue, human lung cancer tissue, mouse liver tissue, mouse kidney tissue, human pancreas cancer tissue, mouse small intestine tissue, rat skin tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28568-1-AP targets CENPF in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPF fusion protein Ag29665
Full Name centromere protein F, 350/400ka (mitosin)
Calculated Molecular Weight 368 kDa
Observed Molecular Weight350 kDa
GenBank Accession NumberNM_016343
Gene Symbol CENPF
Gene ID (NCBI) 1063
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).


Product Specific Protocols
WB protocol for CENPF antibody 28568-1-APDownload protocol
IHC protocol for CENPF antibody 28568-1-APDownload protocol
IF protocol for CENPF antibody 28568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Transl Androl Urol

CENPF promotes the proliferation of renal cell carcinoma in vitro

Authors - Ji Zhang
  • KD Validated

Nat Commun

CSTF2 mediated mRNA N6-methyladenosine modification drives pancreatic ductal adenocarcinoma m6A subtypes

Authors - Yanfen Zheng

Oncol Lett

FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma

Authors - Peipei Li