CoraLite® Plus 488-conjugated CENPF Polyclonal antibody

CENPF Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28568

Synonyms

AH antigen, CENF, CENP F, CENPF, Centromere protein F, hcp 1, Kinetochore protein CENPF, Mitosin, PRO1779

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Validation Data Gallery

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  IF Staining of A549 using CL488-28568

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 CENPF antibody (CL488-28568) at dilution of 1:200.

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  IF Staining of HeLa using CL488-28568

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CENPF antibody (CL488-28568) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: A549 cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28568 targets CENPF in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CENPF fusion protein Ag29665
• Full Name: centromere protein F, 350/400ka (mitosin)
• Calculated Molecular Weight: 368 kDa
• Observed Molecular Weight: 350 kDa
• GenBank Accession Number: NM_016343
• Gene Symbol: CENPF
• Gene ID (NCBI): 1063
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).