CERK Polyclonal antibody

CERK Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25731-1-AP

Synonyms

Acylsphingosine kinase, ceramide kinase, CERK, dA59H18.2, dA59H18.3, hCERK, KIAA1646, Lipid kinase 4, LK4



Tested Applications

Positive WB detected inmouse heart tissue, mouse liver tissue
Positive IHC detected inmouse heart tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25731-1-AP is CERK Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CERK fusion protein Ag22774
Full Name ceramide kinase
Calculated molecular weight 537 aa, 60 kDa, 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC126940
Gene symbol CERK
Gene ID (NCBI) 64781
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ceramide kinase (CERK) is a crucial lipid kinase that specifically catalyses ceramide (CER) to ceramide-1-phosphate. It plays a vital role in biological functions and certain diseases, including aberrant wound healing (PMID: 24823941), obesity-diabetes (PMID: 22465662), mesangio-proliferativekidney diseases (PMID: 25134723), cancer (PMID: 25164007) and neuroblastoma (PMID: 22579669). CERK has 2 isoforms with the molecular weight of 60 and 38 kDa.

Protocols

Product Specific Protocols
WB protocol for CERK antibody 25731-1-APDownload protocol
IHC protocol for CERK antibody 25731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols