CES1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CES1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 16912-1-AP

Synonyms

ACAT, Brain carboxylesterase hBr1, CE1, CEH, CES1, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

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Validation Data Gallery

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  WB analysis using 16912-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16912-1-AP (CES1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16912-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16912-1-AP (CES1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16912-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16912-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16912-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16912-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16912-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16912-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 16912-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16912-1-AP (CES1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

16912-1-AP targets CES1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CES1 fusion protein Ag10567
• Full Name: carboxylesterase 1 (monocyte/macrophage serine esterase 1)
• Calculated Molecular Weight: 566 aa, 62 kDa
• Observed Molecular Weight: 60-63 kDa
• GenBank Accession Number: BC012418
• Gene Symbol: CES1
• Gene ID (NCBI): 1066
• RRID: AB_2077500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CES1(liver carboxylesterase 1) is also named as SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).

Publications

Species	Application	Title
human	WB	Brain; ATAD3 gene cluster deletions cause cerebellar dysfunction associated with altered mitochondrial DNA and cholesterol metabolism.; Authors - Radha Desai
human	WB,IHC	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou ; KO Validated
human	WB,IF	Oncotarget; Multifactorial resistance to aminopeptidase inhibitor prodrug CHR2863 in myeloid leukemia cells: down-regulation of carboxylesterase 1, drug sequestration in lipid droplets and pro-survival activation ERK/Akt/mTOR.; Authors - Sue Ellen Verbrugge
human	WB	Br J Pharmacol; PMID: 28128444; Authors - Wenjing Luo
human	WB	Drug Metab Dispos; Simultaneous absolute protein quantification of carboxylesterases 1 and 2 in human liver tissue fractions using liquid chromatography-tandem mass spectrometry.; Authors - Sato Yuichiro Y
mouse	WB	J Pharm Pharmacol; Clock gene Bmal1 controls diurnal rhythms in expression and activity of intestinal carboxylesterase 1.; Authors - Xun Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Maria (Verified Customer) (08-15-2021)

CES1 in human primary fibroblasts. 10 ug of protein. Blocked with 5% milk in PBST (0.1% Tween). 1:1000 primary ab O/N incubation at 4ºC. 1:5000 HRP conjugated secondary 2h RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary fibroblasts

CES1 Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:16912-1-AP)