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  • KD/KO Validated

CES1 Polyclonal antibody

CES1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 16912-1-AP


ACAT, Brain carboxylesterase hBr1, CE1, CEH, CES1, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16912-1-AP targets CES1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CES1 fusion protein Ag10567
Full Name carboxylesterase 1 (monocyte/macrophage serine esterase 1)
Calculated Molecular Weight 566 aa, 62 kDa
Observed Molecular Weight 60-63 kDa
GenBank Accession NumberBC012418
Gene Symbol CES1
Gene ID (NCBI) 1066
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CES1(liver carboxylesterase 1) is also named as SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).


Product Specific Protocols
WB protocol for CES1 antibody 16912-1-APDownload protocol
IHC protocol for CES1 antibody 16912-1-APDownload protocol
IF protocol for CES1 antibody 16912-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




ATAD3 gene cluster deletions cause cerebellar dysfunction associated with altered mitochondrial DNA and cholesterol metabolism.

Authors - Radha Desai

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
  • KO Validated


Multifactorial resistance to aminopeptidase inhibitor prodrug CHR2863 in myeloid leukemia cells: down-regulation of carboxylesterase 1, drug sequestration in lipid droplets and pro-survival activation ERK/Akt/mTOR.

Authors - Sue Ellen Verbrugge

Br J Pharmacol

PMID: 28128444

Authors - Wenjing Luo

Drug Metab Dispos

Simultaneous absolute protein quantification of carboxylesterases 1 and 2 in human liver tissue fractions using liquid chromatography-tandem mass spectrometry.

Authors - Sato Yuichiro Y

J Pharm Pharmacol

Clock gene Bmal1 controls diurnal rhythms in expression and activity of intestinal carboxylesterase 1.

Authors - Xun Chen


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Maria (Verified Customer) (08-15-2021)

CES1 in human primary fibroblasts. 10 ug of protein. Blocked with 5% milk in PBST (0.1% Tween). 1:1000 primary ab O/N incubation at 4ºC. 1:5000 HRP conjugated secondary 2h RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human primary fibroblasts
CES1 Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:16912-1-AP)