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hCG Beta Monoclonal antibody

hCG Beta Monoclonal Antibody for IHC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D6B8

Cat no : 60334-1-Ig

Synonyms

CG beta, CGB, CGB,hCG, CGB3, CGB5, CGB7, CGB8, hCG, hCGB

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Beta Monoclonal antibody (60334-1-Ig)

    IHC staining of human placenta using 60334-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60334-1-Ig (hCG beta antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Beta Monoclonal antibody (60334-1-Ig)

    IHC staining of human placenta using 60334-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60334-1-Ig (hCG beta antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60334-1-Ig targets hCG Beta in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen hCG Beta fusion protein Ag2191
Full Name CG beta
Calculated Molecular Weight 165 aa, 18 kDa
GenBank Accession NumberBC022796
Gene Symbol CGB
Gene ID (NCBI) 1082
RRIDAB_2881443
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CGB is a member of the glycoprotein hormone beta chain family. Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.

Protocols

Product Specific Protocols
IHC protocol for hCG Beta antibody 60334-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Placenta

Loss of selenocysteine insertion sequence binding protein 2 suppresses the proliferation, migration/invasion and hormone secretion of human trophoblast cells via the PI3K/Akt and ERK signaling pathway.

Authors - Mengdi Li
View Article