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  • KD/KO Validated

CHAF1A Polyclonal antibody

CHAF1A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 17037-1-AP


CAF, CAF 1, CAF 1 subunit A, CAF I 150 kDa subunit, CAF I p150, CAF1, CAF1B, CAF1P150, CHAF1A, hp150, P150

Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman cervix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17037-1-AP targets CHAF1A in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHAF1A fusion protein Ag10536
Full Name chromatin assembly factor 1, subunit A (p150)
Calculated Molecular Weight 956 aa, 107 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC067093
Gene Symbol CHAF1A
Gene ID (NCBI) 10036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromatin assembly factor 1 (CAF1) is the only histone chaperone known to assemble histones H3 and H4 onto newly synthesized DNA both in vitro and in vivo [PMID:17065558]. The 938 amino acid multidomain p150 (CHAF1A) binds via its C-terminal third to p60, which is an essential step for nucleosome assembly because knocking down either subunit disrupts the activity [PMID:14519857]. In addition, CAF1 facilitates DNA synthesis depending on the binding of the N-terminal 31 residues of p150 to the proliferating cell nuclear antigen (PCNA), which acts as a sliding clamp to stimulate the processivity of DNA polymerase [PMID:10648606]. CHAF1A regulates the formation of heterochromatin in mammalian cells during replication and in plants it maintains the transcription of certain subsets of genes. Furthermore, CHAF1A exists in a chromatin-remodeling complex WINAC, which coactivates ligand-induced transactivation function of the vitamin D receptor [PMID:12837248]. CHAF1A protein exists some phosphorylation sites, which may affect its theoretical molecular weight when tested.And a 150 kDa band was recognized (PMID:27445493).


Product Specific Protocols
WB protocol for CHAF1A antibody 17037-1-APDownload protocol
IHC protocol for CHAF1A antibody 17037-1-APDownload protocol
IP protocol for CHAF1A antibody 17037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Struct Mol Biol

Reprogramming of the histone H3.3 landscape in the early mouse embryo.

Authors - Takashi Ishiuchi


Histone chaperone CAF-1 promotes HIV-1 latency by leading the formation of phase-separated suppressive nuclear bodies.

Authors - Xiancai Ma
  • KO Validated

Cancers (Basel)

Human Ccr4 and Caf1 Deadenylases Regulate Proliferation and Tumorigenicity of Human Gastric Cancer Cells via Modulating Cell Cycle Progression.

Authors - Xiao-Hui Song
  • KD Validated

J Biol Chem

The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.

Authors - Nasim A Begum

Front Mol Biosci

An integrated analysis of prognostic mRNA signature in early- and progressive-stage gastric adenocarcinoma

Authors - Xiaoling Hong


Co-Expression of Chromatin Assembly Factor 1 Subunit A and Proliferating Cell Nuclear Antigen Is a Prognostic Biomarker of Esophageal Cancer

Authors - Bing Wen


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Hasan (Verified Customer) (05-17-2023)

HeLa cells were pre-extracted for 5 mins then fixed with 4% PFA for 15 mins. Blocking with 5% FBS for 40 min and primary antibody incubation overnight.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: HeLa cells
CHAF1A Antibody Immunofluorescence validation (1:300 dilution) in HeLa cells (Cat no:17037-1-AP)