Validation Data Gallery
|Positive WB detected in||Jurkat cells, HeLa cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human cervix tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17037-1-AP targets CHAF1A in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHAF1A fusion protein Ag10536|
|Full Name||chromatin assembly factor 1, subunit A (p150)|
|Calculated molecular weight||956 aa, 107 kDa|
|Observed molecular weight||150 kDa|
|GenBank accession number||BC067093|
|Gene ID (NCBI)||10036|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Chromatin assembly factor 1 (CAF1) is the only histone chaperone known to assemble histones H3 and H4 onto newly synthesized DNA both in vitro and in vivo [PMID:17065558]. The 938 amino acid multidomain p150 (CHAF1A) binds via its C-terminal third to p60, which is an essential step for nucleosome assembly because knocking down either subunit disrupts the activity [PMID:14519857]. In addition, CAF1 facilitates DNA synthesis depending on the binding of the N-terminal 31 residues of p150 to the proliferating cell nuclear antigen (PCNA), which acts as a sliding clamp to stimulate the processivity of DNA polymerase [PMID:10648606]. CHAF1A regulates the formation of heterochromatin in mammalian cells during replication and in plants it maintains the transcription of certain subsets of genes. Furthermore, CHAF1A exists in a chromatin-remodeling complex WINAC, which coactivates ligand-induced transactivation function of the vitamin D receptor [PMID:12837248]. CHAF1A protein exists some phosphorylation sites, which may affect its theoretical molecular weight when tested.And a 150 kDa band was recognized (PMID:27445493).
|Product Specific Protocols|
|WB protocol for CHAF1A antibody 17037-1-AP||Download protocol|
|IHC protocol for CHAF1A antibody 17037-1-AP||Download protocol|
|IF protocol for CHAF1A antibody 17037-1-AP||Download protocol|
|IP protocol for CHAF1A antibody 17037-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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