Tested Applications
| Positive IHC detected in | mouse pancreas tissue, human lung cancer tissue, human small intestine tissue, human colon tissue, mouse colon tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse small intestine tissue |
| Positive IF-Fro detected in | mouse small intestine tissue |
| Positive IF/ICC detected in | Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23342-1-AP targets Chromogranin A in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19780 Product name: Recombinant human CHGA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 19-457 aa of BC006459 Sequence: LPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG Predict reactive species |
| Full Name | chromogranin A (parathyroid secretory protein 1) |
| Calculated Molecular Weight | 51 kDa |
| GenBank Accession Number | BC006459 |
| Gene Symbol | Chromogranin A |
| Gene ID (NCBI) | 1113 |
| RRID | AB_2879259 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10645 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Chromogranin A antibody 23342-1-AP | Download protocol |
| IHC protocol for Chromogranin A antibody 23342-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Chromogranin A polyclonal antibody (Cat# 23342-1-AP) has 13 citations spanning journals including Nature Biotechnology, Advanced Science, Developmental Cell, EMBO Journal, PLoS Biology, Journal of Translational Medicine, Frontiers in Immunology, International Journal of Biological Macromolecules, Cell Molecular Gastroenterology and Hepatology, International Journal of Molecular Sciences, Journal of Dairy Science, Cell Biology International, and Prostate. Research fields cover neuroendocrine biology, intestinal stem cell biology, organoid modeling, cancer (lung, prostate, neuroblastoma), gastric preneoplasia, and inflammation.
| Species | Application | Title |
|---|---|---|
Nat Biotechnol A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels. | ||
Adv Sci (Weinh) A Non-Canonical Core Transcriptional Regulatory Circuit Orchestrates Chromatin Reprogramming to Drive Osimertinib Resistance in Non-Small Cell Lung Cancer. | ||
J Transl Med SLIT3-mediated intratumoral crosstalk induces neuroblastoma differentiation via a spontaneous regression-like program | ||
Dev Cell Neuroendocrine control of intestinal regeneration through the vascular niche in Drosophila. | ||
Int J Biol Macromol Digestive and fermentative characteristics of B-type maize starch associated with microbiota-metabolite remodeling and enhanced intestinal stem cell activity. | ||
