Validation Data Gallery
|Positive WB detected in||PC-12 cells, RAW 264.7 cells, mouse brain tissue|
|Positive IHC detected in||human pancreas tissue, mouse pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-12 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14968-1-AP targets Chromogranin B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Chromogranin B fusion protein Ag6833|
|Full Name||chromogranin B (secretogranin 1)|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC000375|
|Gene ID (NCBI)||1114|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Biosci Biotechnol Biochem
Identification of proteins that bind to the neuroprotective agent neoechinulin A.
Citrobacter rodentium Infection Induces Persistent Molecular Changes and Interferon Gamma-Dependent Major Histocompatibility Complex Class II Expression in the Colonic Epithelium.
J Proteome Res
Label-Free LC-MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis.
Kaposi's Sarcoma-Associated Herpesvirus Infection Induces the Expression of Neuroendocrine Genes in Endothelial Cells.
J Cell Sci
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Julia (Verified Customer) (03-14-2022)
Works very well after 1 hour incubation in PBS-T supplemented with normal donkey serum (secondary also added for an hour). Very clear staining.