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  • KD/KO Validated

Chromogranin B Polyclonal antibody

Chromogranin B Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14968-1-AP


CgB, CHGB, Chromogranin B, SCG1, Secretogranin 1, Secretogranin I, SgI

Tested Applications

Positive WB detected inNIH/3T3 cells, mouse brain tissue, RAW 264.7 cells
Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells, mouse colon tissue
Positive FC detected inPC-12 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14968-1-AP targets Chromogranin B in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chromogranin B fusion protein Ag6833
Full Name chromogranin B (secretogranin 1)
Calculated molecular weight 78 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000375
Gene symbol CHGB
Gene ID (NCBI) 1114
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for Chromogranin B antibody 14968-1-APDownload protocol
IHC protocol for Chromogranin B antibody 14968-1-APDownload protocol
IF protocol for Chromogranin B antibody 14968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Cell Sci

PMID: 31182646

Authors - Shelby C Bearrows

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Authors - Caroline Mullineaux-Sanders

Cell Res

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Julia (Verified Customer) (03-14-2022)

Works very well after 1 hour incubation in PBS-T supplemented with normal donkey serum (secondary also added for an hour). Very clear staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Mouse colonic tissue