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  • KD/KO Validated

Chromogranin B Polyclonal antibody

Chromogranin B Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14968-1-AP

Synonyms

CgB, CHGB, Chromogranin B, SCG1, Secretogranin 1, Secretogranin I, SgI



Tested Applications

Positive WB detected inNIH/3T3 cells, mouse brain tissue, RAW 264.7 cells
Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells, mouse colon tissue
Positive FC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14968-1-AP targets Chromogranin B in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chromogranin B fusion protein Ag6833
Full Name chromogranin B (secretogranin 1)
Calculated molecular weight 78 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000375
Gene symbol CHGB
Gene ID (NCBI) 1114
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Chromogranin B antibody 14968-1-APDownload protocol
IHC protocol for Chromogranin B antibody 14968-1-APDownload protocol
IF protocol for Chromogranin B antibody 14968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Cell Sci

PMID: 31182646

Authors - Shelby C Bearrows
humanWB

J Virol

Kaposi's Sarcoma-Associated Herpesvirus Infection Induces the Expression of Neuroendocrine Genes in Endothelial Cells.

Authors - Mohanan Valiya Veettil
humanWB

J Proteome Res

Label-Free LC-MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis.

Authors - Mahlon Angus Collins
mouseWB

Biosci Biotechnol Biochem

Identification of proteins that bind to the neuroprotective agent neoechinulin A.

Authors - Shinji Kamisuki
  • KD Validated
mouseIF

mBio

Citrobacter rodentium Infection Induces Persistent Molecular Changes and Interferon Gamma-Dependent Major Histocompatibility Complex Class II Expression in the Colonic Epithelium.

Authors - Caroline Mullineaux-Sanders
mouseIF

Cell Res

Famsin, a novel gut-secreted hormone, contributes to metabolic adaptations to fasting via binding to its receptor OLFR796

Authors - Aijun Long

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Julia (Verified Customer) (03-14-2022)

Works very well after 1 hour incubation in PBS-T supplemented with normal donkey serum (secondary also added for an hour). Very clear staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Mouse colonic tissue