Validation Data Gallery
|Positive WB detected in||NIH/3T3 cells, mouse brain tissue, RAW 264.7 cells|
|Positive IHC detected in||human pancreas tissue, mouse pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-12 cells, mouse colon tissue|
|Positive FC detected in||PC-12 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14968-1-AP targets Chromogranin B in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Chromogranin B fusion protein Ag6833|
|Full Name||chromogranin B (secretogranin 1)|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC000375|
|Gene ID (NCBI)||1114|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
J Cell Sci
Kaposi's Sarcoma-Associated Herpesvirus Infection Induces the Expression of Neuroendocrine Genes in Endothelial Cells.
J Proteome Res
Label-Free LC-MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis.
Biosci Biotechnol Biochem
Identification of proteins that bind to the neuroprotective agent neoechinulin A.
Citrobacter rodentium Infection Induces Persistent Molecular Changes and Interferon Gamma-Dependent Major Histocompatibility Complex Class II Expression in the Colonic Epithelium.
Famsin, a novel gut-secreted hormone, contributes to metabolic adaptations to fasting via binding to its receptor OLFR796
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Julia (Verified Customer) (03-14-2022)
Works very well after 1 hour incubation in PBS-T supplemented with normal donkey serum (secondary also added for an hour). Very clear staining.