CHKB Polyclonal antibody

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  WB analysis using 22066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22066-1-AP (CHKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CHKB Polyclonal Antibody for WB, ELISA

Cat No. 22066-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EK, CKEKB, CKB, CK, CHKL

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Tested Applications

• Positive WB detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22066-1-AP targets CHKB in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17142

  Product name: Recombinant human CHKB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-102 aa of BC101488

  Sequence: MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRDAERRAYQWCREYLGGAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVL

• Full Name: choline kinase beta
• Calculated Molecular Weight: 395 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC101488
• Gene Symbol: CHKB
• Gene ID (NCBI): 1120
• RRID: AB_3669385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y259
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.