CISD1 Monoclonal antibody

CISD1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, chicken, rabbit

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

1D7E3

Cat no : 68030-1-Ig

Synonyms

C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, ZCD1



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, pig brain tissue, rabbit brain tissue, rat brian tissue, mouse brain tissue, chicken brain tissue, mouse cerebellum tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

68030-1-Ig targets CISD1 in WB, IHC applications and shows reactivity with Human, mouse, rat, chicken, rabbit samples.

Tested Reactivity Human, mouse, rat, chicken, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CISD1 fusion protein Ag8560
Full Name CDGSH iron sulfur domain 1
Calculated molecular weight 108 aa, 12 kDa
Observed molecular weight 14-17 kDa
GenBank accession numberBC007043
Gene symbol CISD1
Gene ID (NCBI) 55847
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.

Protocols

Product Specific Protocols
WB protocol for CISD1 antibody 68030-1-IgDownload protocol
IHC protocol for CISD1 antibody 68030-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols