CoraLite® Plus 488-conjugated CISD1 Monoclonal antibody

CISD1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, chicken

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D7E3

Cat no : CL488-68030

Synonyms

EC:2.6.1.3, Cysteine transaminase CISD1, CDGSH iron-sulfur domain-containing protein 1, CDGSH iron sulfur domain 1, C10orf70

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Validation Data Gallery

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  IF Staining of H9C2 using CL488-68030

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using CoraLite® Plus 488 CISD1 antibody (CL488-68030, Clone: 1D7E3 ) at dilution of 1:200, CL594-phalloidin (red).

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  FC experiment of HeLa using CL488-68030

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CISD1 Monoclonal antibody (CL488-68030, Clone:1D7E3)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: H9C2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68030 targets CISD1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, rabbit, chicken samples.

• Tested Reactivity: human, mouse, rat, rabbit, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CISD1 fusion protein Ag8560
• Full Name: CDGSH iron sulfur domain 1
• Calculated Molecular Weight: 108 aa, 12 kDa
• Observed Molecular Weight: 14-17 kDa
• GenBank Accession Number: BC007043
• Gene Symbol: CISD1
• Gene ID (NCBI): 55847
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.