CLEC4G Polyclonal antibody

CLEC4G Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18173-1-AP

Synonyms

CLEC4G, LP2698, LSECtin, UNQ431

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Validation Data Gallery

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  WB analysis of HUVEC using 18173-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 18173-1-AP (CLEC4G Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human tonsillitis using 18173-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18173-1-AP (CLEC4G Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 18173-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18173-1-AP (CLEC4G Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HUVEC cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18173-1-AP is CLEC4G Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLEC4G fusion protein Ag12837
• Full Name: C-type lectin domain family 4, member G
• Calculated molecular weight: 293 aa, 33 kDa
• Observed molecular weight: 40 kDa
• GenBank accession number: BC093691
• Gene symbol: CLEC4G
• Gene ID (NCBI): 339390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CLEC4G, also known as LSECtin, is a member of the C-type lectin family of glycan-binding receptors that is expressed on sinusoidal endothelial cells in liver and lymph nodes. CLEC4G is a type II transmembrane protein of ∼40 kDa in size with a single C-type lectin-like domain at the COOH terminus, closest in homology to DC-SIGNR, DC-SIGN, and CD23. CLEC4G has been reported as a receptor for Japanese encephalitis virus (PubMed:24623090), ebolavirus (PubMed:16051304), SARS coronavirus/SARS-CoV (PubMed:16051304), lassa virus and Lymphocytic choriomeningitis virus glycoprotein (PubMed:22156524, PubMed:22673088).