CLINT1 Polyclonal antibody

CLINT1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 10470-1-AP

Synonyms

clathrin interactor 1, CLINT, CLINT1, ENTH, Enthoprotin, EPN4, EPNR, Epsin 4, Epsin related protein, EPSINR, KIAA0171



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10470-1-AP targets CLINT1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLINT1 fusion protein Ag0754
Full Name clathrin interactor 1
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa, 80 kDa
GenBank accession numberBC004467
Gene symbol CLINT1
Gene ID (NCBI) 9685
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clathrin interactor 1 (CLINT1), also known as enthoprotin or epsin-4, belongs to the epsin family of endocytic adapter proteins. CLINT1 interacts with clathrin, the adapter protein AP-1 and phosphoinositides. It may have a role in the formation of clathrin coated vesicles and trafficking between the trans-Golgi network and endosomes. Mutations in the gene of CLINT1 are associated with a susceptibility to schizophrenia and psychotic disorders. Calculated molecular weight of CLINT1 is 68 kDa. The migration of endogenous CLINT1 at 80 kDa has also been reported (PMID: 12213833; 15811338).

Protocols

Product Specific Protocols
WB protocol for CLINT1 antibody 10470-1-APDownload protocol
IHC protocol for CLINT1 antibody 10470-1-APDownload protocol
IF protocol for CLINT1 antibody 10470-1-APDownload protocol
IP protocol for CLINT1 antibody 10470-1-APDownload protocol
FC protocol for CLINT1 antibody 10470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-16-2019)

Recognizes CLINT1 prominently with some background, non-specific bands that were of lower intensity than CLINT1.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MDCK GII
CLINT1 Antibody Western Blot, validation (1:1000 dilution) in MDCK GII (Cat no:10470-1-AP)