CLINT1 Polyclonal antibody
CLINT1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10470-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, mouse brain tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Product Information
10470-1-AP targets CLINT1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CLINT1 fusion protein Ag0754 |
Full Name | clathrin interactor 1 |
Calculated molecular weight | 68 kDa |
Observed molecular weight | 68 kDa, 80 kDa |
GenBank accession number | BC004467 |
Gene symbol | CLINT1 |
Gene ID (NCBI) | 9685 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Clathrin interactor 1 (CLINT1), also known as enthoprotin or epsin-4, belongs to the epsin family of endocytic adapter proteins. CLINT1 interacts with clathrin, the adapter protein AP-1 and phosphoinositides. It may have a role in the formation of clathrin coated vesicles and trafficking between the trans-Golgi network and endosomes. Mutations in the gene of CLINT1 are associated with a susceptibility to schizophrenia and psychotic disorders. Calculated molecular weight of CLINT1 is 68 kDa. The migration of endogenous CLINT1 at 80 kDa has also been reported (PMID: 12213833; 15811338).
Protocols
Product Specific Protocols | |
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WB protocol for CLINT1 antibody 10470-1-AP | Download protocol |
IHC protocol for CLINT1 antibody 10470-1-AP | Download protocol |
IF protocol for CLINT1 antibody 10470-1-AP | Download protocol |
IP protocol for CLINT1 antibody 10470-1-AP | Download protocol |
FC protocol for CLINT1 antibody 10470-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Joleen (Verified Customer) (06-16-2019) | Recognizes CLINT1 prominently with some background, non-specific bands that were of lower intensity than CLINT1.
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