CLINT1 Polyclonal antibody

CLINT1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 10470-1-AP

Synonyms

clathrin interactor 1, CLINT, CLINT1, ENTH, Enthoprotin, EPN4, EPNR, Epsin 4, Epsin related protein, EPSINR, KIAA0171

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Validation Data Gallery

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  WB analysis of HeLa using 10470-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10470-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10470-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10470-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10470-1-AP

  IP Result of anti-CLINT1 (IP:10470-1-AP, 4ug; Detection:10470-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 10470-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10470-1-AP (CLINT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10470-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10470-1-AP (CLINT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10470-1-AP

  Immunofluorescent analysis of HepG2 cells, using CLINT1antibody 10470-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 10470-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug CLINT1 antibody (10470-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Product Information

10470-1-AP targets CLINT1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLINT1 fusion protein Ag0754
• Full Name: clathrin interactor 1
• Calculated molecular weight: 68 kDa
• Observed molecular weight: 68 kDa, 80 kDa
• GenBank accession number: BC004467
• Gene symbol: CLINT1
• Gene ID (NCBI): 9685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clathrin interactor 1 (CLINT1), also known as enthoprotin or epsin-4, belongs to the epsin family of endocytic adapter proteins. CLINT1 interacts with clathrin, the adapter protein AP-1 and phosphoinositides. It may have a role in the formation of clathrin coated vesicles and trafficking between the trans-Golgi network and endosomes. Mutations in the gene of CLINT1 are associated with a susceptibility to schizophrenia and psychotic disorders. Calculated molecular weight of CLINT1 is 68 kDa. The migration of endogenous CLINT1 at 80 kDa has also been reported (PMID: 12213833; 15811338).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-16-2019)

Recognizes CLINT1 prominently with some background, non-specific bands that were of lower intensity than CLINT1.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MDCK GII

CLINT1 Antibody Western Blot, validation (1:1000 dilution) in MDCK GII (Cat no:10470-1-AP)

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