CLIP1 Polyclonal antibody, PBS Only

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  WB analysis using 23839-1-AP (same clone as 23839-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23839-1-AP (same clone as 23839-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 23839-1-AP (same clone as 23839-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 23839-1-AP (same clone as 23839-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 23839-1-AP (same clone as 23839-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 23839-1-AP (same clone as 23839-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 23839-1-AP (same clone as 23839-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 23839-1-AP (same clone as 23839-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 23839-1-AP (same clone as 23839-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 23839-1-AP (same clone as 23839-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 23839-1-AP (same clone as 23839-1-PBS)

  IP result of anti-CLIP1 (IP:23839-1-AP, 4ug; Detection:23839-1-AP 1:2000) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 23839-1-AP (same clone as 23839-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CLIP1 antibody (23839-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 23839-1-PBS in a different storage buffer formulation.

CLIP1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 23839-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

CAP-Gly domain-containing linker protein 1, CLIP-170, CYLN1, Cytoplasmic linker protein 170 alpha-2, Reed-Sternberg intermediate filament-associated protein

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Product Information

23839-1-PBS targets CLIP1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20811

  Product name: Recombinant human CLIP1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1090-1438 aa of BC126305

  Sequence: DAMQIMEQMTKEKTETLASLEDTKQTNAKLQNELDTLKENNLKNVEELNKSKELLTVENQKMEEFRKEIETLKQAAAQKSQQLSALQEENVKLAEELGRSRDEVTSHQKLEEERSVLNNQLLEMKKRESKFIKDADEEKASLQKSISITSALLTEKDAELEKLRNEVTVLRGENASAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDETF

• Full Name: CAP-GLY domain containing linker protein 1
• Calculated Molecular Weight: 1438 aa, 162 kDa
• Observed Molecular Weight: 160-170 kDa, 135 kDa
• GenBank Accession Number: BC126305
• Gene Symbol: CLIP1
• Gene ID (NCBI): 6249
• RRID: AB_2879334
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P30622
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CLIP1, also known as RSN, CYLN1, is a member of the microtubule (MT) plus-end tracking protein family. CLIP1 associates with the ends of growing MTs. These proteins regulate MT dynamic behavior and are important for MT-mediated transport over the length of axons and dendrites. It has an important role in spermatogenesis as well as in neuronal development(PMID: 24569606).