CLIP1 Polyclonal antibody

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• CLIP1 Polyclonal antibody (23839-1-AP)

CLIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 23839-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Synonyms

CAP-Gly domain-containing linker protein 1, CLIP-170, CYLN1, Cytoplasmic linker protein 170 alpha-2, Reed-Sternberg intermediate filament-associated protein

• 

  WB analysis using 23839-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 23839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 23839-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat kidney using 23839-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U-937 using 23839-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 23839-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 23839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 23839-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 23839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 23839-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 23839-1-AP

  IP result of anti-CLIP1 (IP:23839-1-AP, 4ug; Detection:23839-1-AP 1:2000) with HeLa cells lysate 2400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 23839-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CLIP1 antibody (23839-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue, U-937 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23839-1-AP targets CLIP1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20811

  Product name: Recombinant human CLIP1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1090-1438 aa of BC126305

  Sequence: DAMQIMEQMTKEKTETLASLEDTKQTNAKLQNELDTLKENNLKNVEELNKSKELLTVENQKMEEFRKEIETLKQAAAQKSQQLSALQEENVKLAEELGRSRDEVTSHQKLEEERSVLNNQLLEMKKRESKFIKDADEEKASLQKSISITSALLTEKDAELEKLRNEVTVLRGENASAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDETF

• Full Name: CAP-GLY domain containing linker protein 1
• Calculated Molecular Weight: 1438 aa, 162 kDa
• Observed Molecular Weight: 160-170 kDa, 135 kDa
• GenBank Accession Number: BC126305
• Gene Symbol: CLIP1
• Gene ID (NCBI): 6249
• RRID: AB_2879334
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P30622
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLIP1, also known as RSN, CYLN1, is a member of the microtubule (MT) plus-end tracking protein family. CLIP1 associates with the ends of growing MTs. These proteins regulate MT dynamic behavior and are important for MT-mediated transport over the length of axons and dendrites. It has an important role in spermatogenesis as well as in neuronal development(PMID: 24569606).

Publications

What published studies show

The anti-CLIP1 antibody (Cat# 23839-1-AP) has 10 total citations across journals including Science, Science Bulletin, Experimental & Molecular Medicine, Cell Death & Differentiation, Cell Death & Disease, Ecotoxicology and Environmental Safety, Frontiers in Cell and Developmental Biology, Scientific Reports, and bioRxiv. Research fields span oocyte spindle assembly, mitotic orientation, breast and cervical cancer, ischemia–reperfusion injury, spermiogenesis, exosomal regulation of airway disease, and cytoskeletal proteomics.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human, mouse	IF	Sci Bull (Beijing); The mechanisms of MTOCs maturation in human and mouse oocytes.; Authors - Hao Gu
rat,human	WB,ELISA	Exp Mol Med; Hypothermic oxygenated perfusion inhibits CLIP1-mediated TIRAP ubiquitination via TFPI2 to reduce ischemia‒reperfusion injury of the fatty liver; Authors - Pengpeng Yue
		Cell Death Differ; USP21-mediated deubiquitylation stimulates NuMA recruitment to the cell cortex to promote mitotic spindle orientation.; Authors - Mingzheng Hu
human	WB	Cell Death Dis; Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration; Authors - Yunfeng Hu
mouse	WB	Ecotoxicol Environ Saf; PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice.; Authors - Chuan Wang