CLIP1 Polyclonal antibody

CLIP1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 23839-1-AP

Synonyms

CLIP, CLIP 170, CLIP1, CLIP170, CYLN1, Cytoplasmic linker protein 1, Restin, RSN



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue, U-937 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23839-1-AP is CLIP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLIP1 fusion protein Ag20811
Full Name CAP-GLY domain containing linker protein 1
Calculated molecular weight 1438 aa, 162 kDa
Observed molecular weight 160-170 kDa, 135 kDa
GenBank accession numberBC126305
Gene symbol CLIP1
Gene ID (NCBI) 6249
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CLIP1 antibody 23839-1-APDownload protocol
IF protocol for CLIP1 antibody 23839-1-APDownload protocol
IP protocol for CLIP1 antibody 23839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice.

Authors - Jingjing Zhang
humanWB

Cell Death Dis

Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration

Authors - Yunfeng Hu
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu