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CLN3 Polyclonal antibody

CLN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20386-1-AP

Synonyms

Protein CLN3, JNCL, BTS, BATTENIN, Batten disease protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLN3 Polyclonal antibody (20386-1-AP)

    WB analysis using 20386-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20386-1-AP (CLN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CLN3 Polyclonal antibody (20386-1-AP)

    WB analysis of SH-SY5Y using 20386-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20386-1-AP (CLN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using CLN3 Polyclonal antibody (20386-1-AP)

    IHC staining of human brain using 20386-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20386-1-AP (CLN3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CLN3 Polyclonal antibody (20386-1-AP)

    IHC staining of human brain using 20386-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20386-1-AP (CLN3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CLN3 Polyclonal antibody (20386-1-AP)

    IF Staining of HepG2 using 20386-1-AP

    Immunofluorescent analysis of HepG2 cells, using CLN3 antibody 20386-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inJurkat cells, SH-SY5Y cells, Raji cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20386-1-AP targets CLN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLN3 fusion protein Ag14224
Full Name ceroid-lipofuscinosis, neuronal 3
Calculated Molecular Weight 438 aa, 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC002394
Gene Symbol CLN3
Gene ID (NCBI) 1201
RRIDAB_10694819
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuronal ceroid lipofuscinosis (NCL, or Batten disease) refers to a group of lethal pediatric neurodegenerative diseases originating from mutations in one of the thus far identified 13 CLN genes (Ceroid Lipofuscinosis, Neuronal type; CLN1 to CLN14) (PMID: 25051496). CLN3 is a multi-membrane spanning protein that is involved in microtubule-dependent, anterograde transport of late endosomes and lysosomes. The CLN3 gene is located on chromosome 16p12.1and produces three mRNA splicing variants. The 438-amino-acid CLN3 protein has a calculated molecular weight of 48 kDa. It has been reported that CLN3 can be glycosylated and form homodimeric complex (PMID: 10356317; 17286803).

Protocols

Product Specific Protocols
WB protocol for CLN3 antibody 20386-1-APDownload protocol
IHC protocol for CLN3 antibody 20386-1-APDownload protocol
IF protocol for CLN3 antibody 20386-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols