Tested Applications
| Positive WB detected in | mouse bladder tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue, human pancreas tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 3 publications below |
Product Information
15039-1-AP targets CMTM8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6776 Product name: Recombinant human CMTM8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-173 aa of BC041390 Sequence: MEEPQRARSHTVTTTASSFAENFSTSSSSFAYDREFLRTLHGFLIVAEIVLGLLVWTLIAGTEYFRVPAFGWVMFVAVSYWVLTVFFLIIYITMTYTRIPQVPWTTVGLCFNGSAFVLYLSAAVVDASSVSPERDSHNFNSWAASSFFAFLVTICYAGNTYFSFIAWRSRTIQ Predict reactive species |
| Full Name | CKLF-like MARVEL transmembrane domain containing 8 |
| Calculated Molecular Weight | 20 kDa |
| Observed Molecular Weight | 25-28 kDa |
| GenBank Accession Number | BC041390 |
| Gene Symbol | CMTM8 |
| Gene ID (NCBI) | 152189 |
| RRID | AB_10949191 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZV2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CMTM8, also known as CKLFSF8, is a member of the chemokine-like factor superfamily (CKLFSF). Proteins of this family share similarity with both the chemokine and the transmembrane 4 superfamilies. CMTM8 is an attenuator of EGF-induced signaling (PMID: 16263120). Overexpression of CMTM8 accelerates ligand-induced clearance of EGFR from the cell surface and induces apoptosis via caspase-dependent and -independent pathways (PMID: 17149703). An alternative splice form of CMTM8, CMTM8-v2, arises from a deletion of exon 2. CMTM8-v2 maintains the ability to induce apoptosis (PMID: 17681841).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CMTM8 antibody 15039-1-AP | Download protocol |
| IP protocol for CMTM8 antibody 15039-1-AP | Download protocol |
| WB protocol for CMTM8 antibody 15039-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
DNA Cell Biol Downregulated CMTM8 Correlates with Poor Prognosis in Gastric Cancer Patients. | ||
BMC Gastroenterol Identification and validation of EMT-immune-related prognostic biomarkers CDKN2A, CMTM8 and ILK in colon cancer. | ||
Bioengineered MicroRNA-582-5p promotes triple-negative breast cancer invasion and metastasis by antagonizing CMTM8 | ||
Cancers (Basel) The MAL Family of Proteins: Normal Function, Expression in Cancer, and Potential Use as Cancer Biomarkers |
