CMTM8 Polyclonal antibody

CMTM8 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15039-1-AP

Synonyms

CKLFSF8, CKLFSF8 V2, CMTM8

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Validation Data Gallery

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  WB analysis of mouse liver using 15039-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15039-1-AP (CMTM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15039-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15039-1-AP (CMTM8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15039-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15039-1-AP (CMTM8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15039-1-AP

  IP result of anti-CMTM8 (IP:15039-1-AP, 4ug; Detection:15039-1-AP 1:300) with mouse liver tissue lysate 6800ug.

• 

  IHC staining of human liver using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse pancreas tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue, human pancreas tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

15039-1-AP targets CMTM8 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CMTM8 fusion protein Ag6776
• Full Name: CKLF-like MARVEL transmembrane domain containing 8
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC041390
• Gene Symbol: CMTM8
• Gene ID (NCBI): 152189
• RRID: AB_10949191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CMTM8, also known as CKLFSF8, is a member of the chemokine-like factor superfamily (CKLFSF). Proteins of this family share similarity with both the chemokine and the transmembrane 4 superfamilies. CMTM8 is an attenuator of EGF-induced signaling (PMID: 16263120). Overexpression of CMTM8 accelerates ligand-induced clearance of EGFR from the cell surface and induces apoptosis via caspase-dependent and -independent pathways (PMID: 17149703). An alternative splice form of CMTM8, CMTM8-v2, arises from a deletion of exon 2. CMTM8-v2 maintains the ability to induce apoptosis (PMID: 17681841).

Publications

Species	Application	Title
human	IHC	DNA Cell Biol; Downregulated CMTM8 Correlates with Poor Prognosis in Gastric Cancer Patients.; Authors - Meng Yan
human	IHC	BMC Gastroenterol; Identification and validation of EMT-immune-related prognostic biomarkers CDKN2A, CMTM8 and ILK in colon cancer.; Authors - Ning Kang
human	WB	Oncol Rep; CMTM8 inhibits the carcinogenesis and progression of bladder cancer.; Authors - Denghui Gao
human	WB	Bioengineered; MicroRNA-582-5p promotes triple-negative breast cancer invasion and metastasis by antagonizing CMTM8; Authors - Xue Zeng
	IHC	Cancers (Basel); The MAL Family of Proteins: Normal Function, Expression in Cancer, and Potential Use as Cancer Biomarkers; Authors - Leticia Labat-de-Hoz

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