CMTM8 Polyclonal antibody

CMTM8 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15039-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Chemokine-like factor superfamily member 8, CKLF-like MARVEL transmembrane domain-containing protein 8, CKLFSF8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse bladder tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human pancreas tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15039-1-AP targets CMTM8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CMTM8 fusion protein Ag6776 Predict reactive species
Full Name CKLF-like MARVEL transmembrane domain containing 8
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC041390
Gene Symbol CMTM8
Gene ID (NCBI) 152189
RRIDAB_10949191
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CMTM8, also known as CKLFSF8, is a member of the chemokine-like factor superfamily (CKLFSF). Proteins of this family share similarity with both the chemokine and the transmembrane 4 superfamilies. CMTM8 is an attenuator of EGF-induced signaling (PMID: 16263120). Overexpression of CMTM8 accelerates ligand-induced clearance of EGFR from the cell surface and induces apoptosis via caspase-dependent and -independent pathways (PMID: 17149703). An alternative splice form of CMTM8, CMTM8-v2, arises from a deletion of exon 2. CMTM8-v2 maintains the ability to induce apoptosis (PMID: 17681841).

Protocols

Product Specific Protocols
WB protocol for CMTM8 antibody 15039-1-APDownload protocol
IHC protocol for CMTM8 antibody 15039-1-APDownload protocol
IP protocol for CMTM8 antibody 15039-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

DNA Cell Biol

Downregulated CMTM8 Correlates with Poor Prognosis in Gastric Cancer Patients.

Authors - Meng Yan
humanIHC

BMC Gastroenterol

Identification and validation of EMT-immune-related prognostic biomarkers CDKN2A, CMTM8 and ILK in colon cancer.

Authors - Ning Kang
humanWB

Oncol Rep

CMTM8 inhibits the carcinogenesis and progression of bladder cancer.

Authors - Denghui Gao
humanWB

Bioengineered

MicroRNA-582-5p promotes triple-negative breast cancer invasion and metastasis by antagonizing CMTM8

Authors - Xue Zeng
IHC

Cancers (Basel)

The MAL Family of Proteins: Normal Function, Expression in Cancer, and Potential Use as Cancer Biomarkers

Authors - Leticia Labat-de-Hoz
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