COA4 Polyclonal antibody

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  WB analysis using 30721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30721-1-AP (COA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30721-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 30721-1-AP (COA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 30721-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30721-1-AP (COA4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COA4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30721-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CHCHD8, E2IG2, E2 induced gene 2 protein

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, PC-3 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30721-1-AP targets COA4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33966

  Product name: Recombinant human CHCHD8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-87 aa of NM_016565.2

  Sequence: MSTSVPQGHTWTQRVKKDDEEEDPLDQLISRSGCAASHFAVQECMAQHQDWRQCQPQVQAFKDCMSEQQARRQEELQRRQEQAGAHH

• Full Name: Cytochrome c oxidase assembly factor 4 homolog, mitochondrial
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: NM_016565.2
• Gene Symbol: COA4
• Gene ID (NCBI): 51287
• RRID: AB_3669754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYJ1-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The coiled-coil-helix-coiled-coil-helix domain containing 8 (CHCHD8) is a putative COX assembly factor known as cytochrome c oxidase assembly factor 4 homolog (CoA4). COA4 is a newly identified CcO assembly factor which is a twin CX(9)C motif mitochondrial protein localized in the intermembrane space linked with the inner membrane of mitochondria. Its transport into intermembrane space depends on the MIA40 trans-site receptor machinery. (PMID: 37345060)