Collagen Type II Polyclonal antibody
Collagen Type II Polyclonal Antibody for IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15943-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 32 publications below |
IHC | See 21 publications below |
IF | See 12 publications below |
Product Information
The immunogen of 15943-1-AP is Collagen Type II Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, pig, rabbit |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Collagen Type II fusion protein Ag8416 |
Full Name | collagen, type II, alpha 1 |
Calculated molecular weight | 134 kDa |
GenBank accession number | BC007252 |
Gene symbol | COL2A1 |
Gene ID (NCBI) | 1280 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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IHC protocol for Collagen Type II antibody 15943-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Autophagy HIF1A Alleviates compression-induced apoptosis of nucleus pulposus derived stem cells via upregulating autophagy. | ||
Redox Biol Advanced oxidation protein products increase TNF-α and IL-1β expression in chondrocytes via NADPH oxidase 4 and accelerate cartilage degeneration in osteoarthritis progression. | ||
Mol Med BMP7 ameliorates intervertebral disc degeneration in type 1 diabetic rats by inhibiting pyroptosis of nucleus pulposus cells and NLRP3 inflammasome activity | ||
Sci Rep Heme oxygenase-1 attenuates IL-1β induced alteration of anabolic and catabolic activities in intervertebral disc degeneration. | ||
Aging (Albany NY) Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis. | ||
Aging (Albany NY) Astaxanthin protects against osteoarthritis via Nrf2: a guardian of cartilage homeostasis. |