Collagen Type II Polyclonal antibody

Collagen Type II Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

54

Cat no : 15943-1-AP

Synonyms

Alpha 1 type II collagen, ANFH, AOM, COL11A3, COL2A1, Collagen alpha 1(II) chain, Collagen Type II, collagen, type II, alpha 1, SEDC

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Validation Data Gallery

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  IHC staining of human lung using 15943-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15943-1-AP (Collagen Type II antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 15943-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15943-1-AP (Collagen Type II antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 32 publications below
• IHC: See 21 publications below
• IF: See 12 publications below

Product Information

The immunogen of 15943-1-AP is Collagen Type II Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type II fusion protein Ag8416
• Full Name: collagen, type II, alpha 1
• Calculated molecular weight: 134 kDa
• GenBank accession number: BC007252
• Gene symbol: COL2A1
• Gene ID (NCBI): 1280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	IHC	Autophagy; HIF1A Alleviates compression-induced apoptosis of nucleus pulposus derived stem cells via upregulating autophagy.; Authors - Ruijun He
human	IHC	Redox Biol; Advanced oxidation protein products increase TNF-α and IL-1β expression in chondrocytes via NADPH oxidase 4 and accelerate cartilage degeneration in osteoarthritis progression.; Authors - Cong-Rui Liao
rat	IHC	Mol Med; BMP7 ameliorates intervertebral disc degeneration in type 1 diabetic rats by inhibiting pyroptosis of nucleus pulposus cells and NLRP3 inflammasome activity; Authors - Xiao-Jun Yu
human	IHC	Sci Rep; Heme oxygenase-1 attenuates IL-1β induced alteration of anabolic and catabolic activities in intervertebral disc degeneration.; Authors - Bo Hu
rat	WB	Aging (Albany NY); Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis.; Authors - Xiaojian Huang
mouse	WB	Aging (Albany NY); Astaxanthin protects against osteoarthritis via Nrf2: a guardian of cartilage homeostasis.; Authors - Kai Sun

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