Collagen Type II Polyclonal antibody

Collagen Type II Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15943-1-AP

Synonyms

Alpha 1 type II collagen, ANFH, AOM, COL11A3, COL2A1, Collagen alpha 1(II) chain, Collagen Type II, collagen, type II, alpha 1, SEDC



Tested Applications

Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 15943-1-AP is Collagen Type II Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type II fusion protein Ag8416
Full Name collagen, type II, alpha 1
Calculated molecular weight 134 kDa
GenBank accession numberBC007252
Gene symbol COL2A1
Gene ID (NCBI) 1280
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for Collagen Type II antibody 15943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Autophagy

HIF1A Alleviates compression-induced apoptosis of nucleus pulposus derived stem cells via upregulating autophagy.

Authors - Ruijun He
humanIHC

Redox Biol

Advanced oxidation protein products increase TNF-α and IL-1β expression in chondrocytes via NADPH oxidase 4 and accelerate cartilage degeneration in osteoarthritis progression.

Authors - Cong-Rui Liao
ratIHC

Mol Med

BMP7 ameliorates intervertebral disc degeneration in type 1 diabetic rats by inhibiting pyroptosis of nucleus pulposus cells and NLRP3 inflammasome activity

Authors - Xiao-Jun Yu
humanIHC

Sci Rep

Heme oxygenase-1 attenuates IL-1β induced alteration of anabolic and catabolic activities in intervertebral disc degeneration.

Authors - Bo Hu
ratWB

Aging (Albany NY)

Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis.

Authors - Xiaojian Huang
mouseWB

Aging (Albany NY)

Astaxanthin protects against osteoarthritis via Nrf2: a guardian of cartilage homeostasis.

Authors - Kai Sun