COL6A6 Polyclonal antibody, PBS Only

• 

  WB analysis using 22302-1-AP (same clone as 22302-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22302-1-AP (COL6A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22302-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 22302-1-AP (same clone as 22302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22302-1-AP (COL6A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22302-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 22302-1-AP (same clone as 22302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22302-1-AP (COL6A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22302-1-PBS in a different storage buffer formulation.

COL6A6 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 22302-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

22302-1-PBS targets COL6A6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: collagen type VI alpha 6
• Calculated Molecular Weight: 247 kDa
• Observed Molecular Weight: 167 kDa, 250 kDa
• GenBank Accession Number: NM_001102608
• Gene Symbol: COL6A6
• Gene ID (NCBI): 131873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A6NMZ7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Collagen type VI alpha 6 chain (COL6A6) belongs to the collagen VI (COL6) family which acts as a vital role in the extracellular matrix. COL6A6 encodes a 2,262-amino acid protein that contains multiple von Willebrand factor domains and forms a component of the basal lamina of epithelial cells (PMID: 33747955). COL6A6 is a novel tumor suppressor gene in NSCLC and can serve as a potentially valuable prognostic biomarker and therapeutic target for NSCLC (PMID: 35116307).