COL6A6 Polyclonal antibody

COL6A6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22302-1-AP

Synonyms

COL6A6, Collagen alpha 6(VI) chain, collagen type VI alpha 6

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Validation Data Gallery

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  WB analysis using 22302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22302-1-AP (COL6A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 22302-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22302-1-AP (COL6A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 22302-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22302-1-AP (COL6A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue
• Positive IHC detected in: mouse heart tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22302-1-AP targets COL6A6 in WB, IHC, ELISA applications and shows reactivity with human, Mouse samples.

• Tested Reactivity: human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: collagen type VI alpha 6
• Calculated Molecular Weight: 247 kDa
• Observed Molecular Weight: 167 kDa, 250 kDa
• GenBank Accession Number: NM_001102608
• Gene Symbol: COL6A6
• Gene ID (NCBI): 131873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen type VI alpha 6 chain (COL6A6) belongs to the collagen VI (COL6) family which acts as a vital role in the extracellular matrix. COL6A6 encodes a 2,262-amino acid protein that contains multiple von Willebrand factor domains and forms a component of the basal lamina of epithelial cells (PMID: 33747955). COL6A6 is a novel tumor suppressor gene in NSCLC and can serve as a potentially valuable prognostic biomarker and therapeutic target for NSCLC (PMID: 35116307).