COQ4 Polyclonal antibody

• Featured Product
• KD/KO Validated

COQ4 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 16654-1-AP

Synonyms

CGI-92, CGI 92

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 16654-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16654-1-AP (COQ4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 16654-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 16654-1-AP (COQ4 Antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16654-1-AP

  IP result of anti-COQ4 (IP:16654-1-AP, 4ug; Detection:16654-1-AP 1:500) with mouse liver tissue lysate 6000 ug.

• 

  IHC staining of human liver using 16654-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16654-1-AP (COQ4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16654-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16654-1-AP (COQ4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 16654-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16654-1-AP (COQ4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 16654-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16654-1-AP (COQ4 Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, Transfected HEK-293 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below

Product Information

16654-1-AP targets COQ4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COQ4 fusion protein Ag10089
• Full Name: coenzyme Q4 homolog (S. cerevisiae)
• Calculated Molecular Weight: 265 aa, 30 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC011895
• Gene Symbol: COQ4
• Gene ID (NCBI): 51117
• RRID: AB_2878296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human coenzyme Q4 (COQ4), as a component of the coenzyme Q biosynthetic pathway, is required for the oxidative decarboxylation of the C1 carbon of Coenzyme Q in eukaryotic cells (PMID: 38295803). COQ4 deficiency manifests as an early-onset neurodegenerative disorder. COQ4 encodes a ubiquitously expressed 265-amino-acid protein that is peripherally associated with the mitochondrial inner membrane on the matrix side (PMID: 25658047, 34656997)

Publications

Species	Application	Title
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB	Redox Biol; The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.; Authors - Pilar González-García
human	WB	Ann Clin Transl Neurol; 4-Hydroxybenzoic acid restores CoQ10 biosynthesis in human COQ2 deficiency.; Authors - Diran Herebian
human	WB	J Med Genet; Human COQ4 deficiency: delineating the clinical, metabolic and neuroimaging phenotypes.; Authors - Lucia Laugwitz
human	WB	Biochim Biophys Acta Bioenerg; Characterization of human mitochondrial PDSS and COQ proteins and their roles in maintaining coenzyme Q10 levels and each other's stability.; Authors - Hsiu-Chuan Yen
human	WB	Mol Genet Metab; Detection of 6-demethoxyubiquinone in CoQ10 deficiency disorders: Insights into enzyme interactions and identification of potential therapeutics.; Authors - Diran Herebian ; KD Validated