COQ6 Polyclonal antibody, PBS Only

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  WB analysis of mouse heart using 12481-1-AP (same clone as 12481-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12481-1-AP (COQ6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12481-1-AP (same clone as 12481-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12481-1-AP (COQ6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 12481-1-AP (same clone as 12481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12481-1-AP (COQ6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 12481-1-AP (same clone as 12481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12481-1-AP (COQ6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12481-1-AP (same clone as 12481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12481-1-AP (COQ6 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

COQ6 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12481-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

12481-1-PBS targets COQ6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3164

  Product name: Recombinant human COQ6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 151-468 aa of BC014181

  Sequence: ALTKQLEAVSDRVTVLYRSKAIRYTWPCPFPMADSSPWVHITLGDGSTFQTKLLIGADGHNSGVRQAVGIQNVSWNYDQSAVVATLHLSEATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAELVSMDEEKFVDAVNSAFWSDADHTDFIDTAGAMLQYAVSLLKPTKVSARQLPPSVARVDAKSRVLFPLGLGHAAEYVRPRVALIGDAAHRVHPLAGQGVNMGFGDISSLAHHLSTAAFNGKDLGSVSHLTGYETERQRHNTALLAATDLLKRLYSTSASPLVLLRTWGLQATNAVSPLKEQIMAFASK

• Full Name: coenzyme Q6 homolog, monooxygenase (S. cerevisiae)
• Calculated Molecular Weight: 468 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC014181
• Gene Symbol: COQ6
• Gene ID (NCBI): 51004
• RRID: AB_10644143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2Z9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

COQ6(Coenzyme Q10 monooxygenase 6) is also named CGI-10 and belongs to the ubiH/COQ6 family. It is an evolutionarily conserved flavin-dependent monooxygenase required for the biosynthesis of coenzyme Q10 or ubiquinone. It has 3 isoforms with a molecular mass of 51 kDa, 43 kDa and 49 kDa. In the inner ear, Coq6 is expressed in the spiral ganglion, stria vascularis cells, and spiral ligament(PMID:21540551 ). Defects in COQ6 are the cause of coenzyme Q10 deficiency, primary, type 6 (COQ10D6).