Tested Applications
| Positive WB detected in | mouse heart tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse ovary tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12481-1-AP targets COQ6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3164 Product name: Recombinant human COQ6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-468 aa of BC014181 Sequence: ALTKQLEAVSDRVTVLYRSKAIRYTWPCPFPMADSSPWVHITLGDGSTFQTKLLIGADGHNSGVRQAVGIQNVSWNYDQSAVVATLHLSEATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAELVSMDEEKFVDAVNSAFWSDADHTDFIDTAGAMLQYAVSLLKPTKVSARQLPPSVARVDAKSRVLFPLGLGHAAEYVRPRVALIGDAAHRVHPLAGQGVNMGFGDISSLAHHLSTAAFNGKDLGSVSHLTGYETERQRHNTALLAATDLLKRLYSTSASPLVLLRTWGLQATNAVSPLKEQIMAFASK Predict reactive species |
| Full Name | coenzyme Q6 homolog, monooxygenase (S. cerevisiae) |
| Calculated Molecular Weight | 468 aa, 51 kDa |
| Observed Molecular Weight | 51 kDa |
| GenBank Accession Number | BC014181 |
| Gene Symbol | COQ6 |
| Gene ID (NCBI) | 51004 |
| RRID | AB_10644143 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2Z9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COQ6(Coenzyme Q10 monooxygenase 6) is also named CGI-10 and belongs to the ubiH/COQ6 family. It is an evolutionarily conserved flavin-dependent monooxygenase required for the biosynthesis of coenzyme Q10 or ubiquinone. It has 3 isoforms with a molecular mass of 51 kDa, 43 kDa and 49 kDa. In the inner ear, Coq6 is expressed in the spiral ganglion, stria vascularis cells, and spiral ligament(PMID:21540551 ). Defects in COQ6 are the cause of coenzyme Q10 deficiency, primary, type 6 (COQ10D6).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for COQ6 antibody 12481-1-AP | Download protocol |
| WB protocol for COQ6 antibody 12481-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The COQ6 Polyclonal antibody (Cat# 12481-1-AP) has 7 citations published in journals including Chin Med J, Antioxidants, EMBO Mol Med, Aging Cell, Oxid Med Cell Longev, and J Assist Reprod Genet. Research fields span coenzyme Q10 biosynthesis, mitochondrial dysfunction, aging, reproductive biology, podocyte injury in glomerulosclerosis, and neurodegeneration. Applications cited include WB, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Chin Med J (Engl) New Mutation of Coenzyme Q10 Monooxygenase 6 Causing Podocyte Injury in a Focal Segmental Glomerulosclerosis Patient. | ||
Antioxidants (Basel) Evolution of COQ-Synthome Transcripts and CoQ Levels in Mice Tissues Along Aging: Effect of Resveratrol and Exercise | ||
Antioxidants (Basel) Co-Enzyme Q10 Supplementation Rescues Cumulus Cells Dysfunction in a Maternal Aging Model. | ||
EMBO Mol Med β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice. | ||
Aging Cell Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging. | ||
Oxid Med Cell Longev Vanillic Acid Restores Coenzyme Q Biosynthesis and ATP Production in Human Cells Lacking COQ6.
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