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  • KD/KO Validated

COQ6 Polyclonal antibody

COQ6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12481-1-AP

Synonyms

CGI 10, COQ6



Tested Applications

Positive WB detected inmouse heart tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse ovary tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12481-1-AP targets COQ6 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COQ6 fusion protein Ag3164
Full Name coenzyme Q6 homolog, monooxygenase (S. cerevisiae)
Calculated Molecular Weight 468 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC014181
Gene Symbol COQ6
Gene ID (NCBI) 51004
RRIDAB_10644143
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COQ6(Coenzyme Q10 monooxygenase 6) is also named CGI-10 and belongs to the ubiH/COQ6 family. It is an evolutionarily conserved flavin-dependent monooxygenase required for the biosynthesis of coenzyme Q10 or ubiquinone. It has 3 isoforms with a molecular mass of 51 kDa, 43 kDa and 49 kDa. In the inner ear, Coq6 is expressed in the spiral ganglion, stria vascularis cells, and spiral ligament(PMID:21540551 ). Defects in COQ6 are the cause of coenzyme Q10 deficiency, primary, type 6 (COQ10D6).

Protocols

Product Specific Protocols
WB protocol for COQ6 antibody 12481-1-APDownload protocol
IHC protocol for COQ6 antibody 12481-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO Mol Med

β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.

Authors - Agustín Hidalgo-Gutiérrez
mouseWB, IF

Aging Cell

Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging.

Authors - Assaf Ben-Meir
humanWB

Oxid Med Cell Longev

Vanillic Acid Restores Coenzyme Q Biosynthesis and ATP Production in Human Cells Lacking COQ6.

Authors - Manuel J Acosta Lopez
  • KO Validated
humanWB

J Assist Reprod Genet

PMID: 26552664

Authors - Yaakov Bentov
humanIHC

Chin Med J (Engl)

New Mutation of Coenzyme Q10 Monooxygenase 6 Causing Podocyte Injury in a Focal Segmental Glomerulosclerosis Patient.

Authors - Cheng-Cheng Song
mouseIF

Antioxidants (Basel)

Co-Enzyme Q10 Supplementation Rescues Cumulus Cells Dysfunction in a Maternal Aging Model.

Authors - Assaf Ben-Meir