COSMC antibody (19254-1-PBS)

Filter: AllWBIHC

WB analysis of HeLa using 19254-1-AP (same clone as 19254-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 19254-1-AP (same clone as 19254-1-PBS)
WB result of COSMC antibody (19254-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COSMC transfected HepG2 cells. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 19254-1-AP (same clone as 19254-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.
WB analysis of Caco-2 using 19254-1-AP (same clone as 19254-1-PBS)
Caco-2 cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 19254-1-AP (same clone as 19254-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 19254-1-AP (same clone as 19254-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19254-1-AP (COSMC antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 19254-1-AP (same clone as 19254-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19254-1-AP (COSMC antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

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Overview

Tested Applications
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Product Information

19254-1-PBS targets COSMC in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	COSMC fusion protein Ag6899 Predict reactive species
Full Name	C1GALT1-specific chaperone 1
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36-37 kDa
GenBank Accession Number	BC050441
Gene Symbol	COSMC
Gene ID (NCBI)	29071
RRID	AB_10638003
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96EU7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

COSMC (C1GALT1C1) is a molecular chaperone required for expression of active T-synthase (CIGALT1) which catalyzes the synthesis of Tn antigen. Tn antigens are cancer-associated glycans and play an important role in tumor progression. Dysfunctional COSMC is able to convert a wild type protein into a tumor-specific antigen affecting tumor cell biology. COSMC overexpression had been observed in various cancers. COSMC mutations have been observed in an autoimmune disease Tn syndrome.