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Carboxypeptidase E Polyclonal antibody

Carboxypeptidase E Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 13710-1-AP


Carboxypeptidase E, Carboxypeptidase H, CPE, CPH, Enkephalin convertase

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pituitary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13710-1-AP targets Carboxypeptidase E in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carboxypeptidase E fusion protein Ag4647
Full Name carboxypeptidase E
Calculated Molecular Weight 476 aa, 53 kDa
Observed Molecular Weight 50 kDa, 52 kDa
GenBank Accession NumberBC033866
Gene Symbol CPE
Gene ID (NCBI) 1363
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.


Product Specific Protocols
WB protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IHC protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IF protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IP protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Metab

Reductive TCA cycle metabolism fuels glutamine- and glucose-stimulated insulin secretion.

Authors - Guo-Fang Zhang


Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.

Authors - Seokwon Jo
human, mouseIHC

Brain Pathol

Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease.

Authors - Virginia Plá

Front Genet

Development and Validation of a Novel Gene Signature for Predicting the Prognosis by Identifying m5C Modification Subtypes of Cervical Cancer.

Authors - Jing Yu
  • KD Validated

Biophys Rep

PMID: 30596141

Authors - Min Li

Function (Oxf)

ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet β-Cell

Authors - Kristen E Rohli