Carboxypeptidase E Polyclonal antibody

Carboxypeptidase E Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13710-1-AP

Synonyms

Carboxypeptidase E, Carboxypeptidase H, CPE, CPH, Enkephalin convertase



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pituitary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13710-1-AP targets Carboxypeptidase E in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carboxypeptidase E fusion protein Ag4647
Full Name carboxypeptidase E
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight 50 kDa, 52 kDa
GenBank accession numberBC033866
Gene symbol CPE
Gene ID (NCBI) 1363
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IHC protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IF protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IP protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biophys Rep

PMID: 30596141

Authors - Min Li
human, mouseIHC

Brain Pathol

Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease.

Authors - Virginia Plá
mouseWB

Diabetes

Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.

Authors - Seokwon Jo
ratWB

Cell Metab

Reductive TCA cycle metabolism fuels glutamine- and glucose-stimulated insulin secretion.

Authors - Guo-Fang Zhang