Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, PC-12 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human pituitary tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse pancreas tissue, mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13710-1-AP targets CPE in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4647 Product name: Recombinant human CPE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 126-476 aa of BC033866 Sequence: FLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELESFSERKEEEKEELMEWWKMMSETLNF Predict reactive species |
| Full Name | carboxypeptidase E |
| Calculated Molecular Weight | 476 aa, 53 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC033866 |
| Gene Symbol | Carboxypeptidase E |
| Gene ID (NCBI) | 1363 |
| RRID | AB_2083913 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16870 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CPE(Carboxypeptidase E) is also named as CPH. It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CPE antibody 13710-1-AP | Download protocol |
| IHC protocol for CPE antibody 13710-1-AP | Download protocol |
| IP protocol for CPE antibody 13710-1-AP | Download protocol |
| WB protocol for CPE antibody 13710-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CPE Polyclonal antibody (catalog number 13710-1-AP) has 17 total citations. These appear in journals including Cell, Cell Metabolism, Translational Neurodegeneration, Cancer Letters, Diabetes, Journal of Cell Biology, Cell Regeneration, Function, Brain Pathology, Scientific Reports, Neuropharmacology, American Journal of Physiology, Neuroimmunomodulation, Frontiers in Genetics, Biophysical Reports, and Heliyon. Research fields span Alzheimer's disease and neurodegeneration, pancreatic β-cell function and insulin secretion, diabetes/glucose homeostasis, cancer (osteosarcoma, cervical and gastric), and neuroinflammation/depression.
| Species | Application | Title |
|---|---|---|
Cell Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2. | ||
Cell Metab Reductive TCA cycle metabolism fuels glutamine- and glucose-stimulated insulin secretion. | ||
Transl Neurodegener Agomirs upregulating carboxypeptidase E expression rescue hippocampal neurogenesis and memory deficits in Alzheimer's disease
| ||
Cancer Lett CPE/HYOU1 axis-activated Hippo-YAP signaling pathway suppresses PANoptosis to facilitate osteosarcoma progression.
| ||
Diabetes Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function. | ||
J Cell Biol Liquid-liquid phase separation facilitates the biogenesis of secretory storage granules |
Reviews
What customers say
The single available review rates the product 5 stars. The user reported successful Western Blot performance on astrocyte conditioned medium samples, using a 1:2000 dilution, and confirmed the antibody works properly for this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Andrea (Verified Customer) (07-24-2026) | It works properly by WB on astrocyte conditioned medium samples
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