|Positive WB detected in||mouse brain tissue, human brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human pituitary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13710-1-AP targets Carboxypeptidase E in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Carboxypeptidase E fusion protein Ag4647|
|Full Name||carboxypeptidase E|
|Calculated molecular weight||476 aa, 53 kDa|
|Observed molecular weight||50 kDa, 52 kDa|
|GenBank accession number||BC033866|
|Gene ID (NCBI)||1363|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.
|Product Specific Protocols|
|WB protocol for Carboxypeptidase E antibody 13710-1-AP||Download protocol|
|IHC protocol for Carboxypeptidase E antibody 13710-1-AP||Download protocol|
|IF protocol for Carboxypeptidase E antibody 13710-1-AP||Download protocol|
|IP protocol for Carboxypeptidase E antibody 13710-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease.
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