CPEB4 Polyclonal antibody

CPEB4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 25342-1-AP

Synonyms

CPE binding protein 4, CPE BP4, CPEB4, hCPEB 4, KIAA1673

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 25342-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25342-1-AP (CPEB4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25342-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25342-1-AP (CPEB4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 25342-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25342-1-AP (CPEB4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 25342-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25342-1-AP (CPEB4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 25342-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25342-1-AP (CPEB4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 25342-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CPEB4 antibody (25342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, A375 cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 2 publications below
• RIP: See 1 publications below

Product Information

25342-1-AP targets CPEB4 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytoplasmic polyadenylation element binding protein 4
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: NM_030627
• Gene Symbol: CPEB4
• Gene ID (NCBI): 80315
• RRID: AB_2880036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPEB4 also named as CPE binding protein 4 or hCPEB 4 is a 729 amino acid protein, which contains two RRM domains and belongs to the RRM CPEB family. CPEB4 involves in some forms of synaptic plasticity and regulate translation by binding to regulatory motifs of defined mRNA targets. CPEB1 and CPEB4 are differentially regulated by phase-specific kinases, generating the need of two sequential CPEB activities to sustain cytoplasmic polyadenylation during all the meiotic phases.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation.; Authors - Kai P Hoefig
human	WB	Front Cell Dev Biol; CPEB4 Inhibit Cell Proliferation via Upregulating p21 mRNA Stability in Renal Cell Carcinoma.; Authors - Jiehui Di
human	WB,IHC	J Transl Med; CircESRP1 enhances metastasis and epithelial-mesenchymal transition in endometrial cancer via the miR-874-3p/CPEB4 axis.; Authors - Rui Shi
human	WB	Technol Cancer Res Treat; miRNA-130a-3p/CPEB4 Axis Modulates Glioblastoma Growth and Progression; Authors - Hongchao Liu
mouse	WB	Acta Cir Bras; Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy.; Authors - Hui Zhang
mouse	WB	bioRxiv; Inhibition of CPEB3 ribozyme elevates CPEB3 protein expression and polyadenylation of its target mRNAs, and enhances object location memory; Authors - Claire C Chen