CPOX Polyclonal antibody, PBS Only

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  WB analysis using 16724-1-AP (same clone as 16724-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 16724-1-AP (same clone as 16724-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 16724-1-AP (same clone as 16724-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16724-1-AP (same clone as 16724-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 16724-1-AP (same clone as 16724-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 16724-1-AP (same clone as 16724-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16724-1-AP (same clone as 16724-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 16724-1-AP (same clone as 16724-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 16724-1-AP (same clone as 16724-1-PBS)

  IP result of anti-CPOX (IP:16724-1-AP, 4ug; Detection:16724-1-AP 1:1000) with HepG2 cells lysate 1800ug. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16724-1-AP (same clone as 16724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16724-1-AP (same clone as 16724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 16724-1-AP (same clone as 16724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 16724-1-AP (same clone as 16724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

CPOX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 16724-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

16724-1-PBS targets CPOX in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10164

  Product name: Recombinant human CPOX protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 95-454 aa of BC017210

  Sequence: HVQRAEMLPKTSGTRATSLGRPEEEEDELAHRCSSFMAPPVTDLGELRRRPGDMKTKMELLILETQAQVCQALAQVDGGANFSVDRWERKEGGGGISCVLQDGCVFEKAGVSISVVHGNLSEEAAKQMRSRGKVLKTKDGKLPFCAMGVSSVIHPKNPHAPTIHFNYRYFEVEEADGHKQWWFGGGCDLTPTYLNQEDAVHFHRTLKEACDQHGPDLYPKFKKWCDDYFFIAHRGERRGIGGIFFDDLDSPSKEEVFRFVQSCARAVVPSYIPLVKKHCDDSFTPQEKLWQQLRRGRYVEFNLLYDRGTKFGLFTPGSRIESILMSLPLTARWEYMHSPSENSKEAEILEVLRHPRDWVR

• Full Name: coproporphyrinogen oxidase
• Calculated Molecular Weight: 454 aa, 50 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC017210
• Gene Symbol: CPOX
• Gene ID (NCBI): 1371
• RRID: AB_2084231
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36551
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.