CPOX Polyclonal antibody

CPOX Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16724-1-AP

Synonyms

Coprogen oxidase, Coproporphyrinogen oxidase, Coproporphyrinogenase, COX, CPO, CPOX, CPX, HCP



Tested Applications

Positive WB detected inHepG2 cells, human liver tissue, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

16724-1-AP targets CPOX in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPOX fusion protein Ag10164
Full Name coproporphyrinogen oxidase
Calculated molecular weight 454 aa, 50 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC017210
Gene symbol CPOX
Gene ID (NCBI) 1371
RRIDAB_2084231
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.

Protocols

Product Specific Protocols
WB protocol for CPOX antibody 16724-1-APDownload protocol
IHC protocol for CPOX antibody 16724-1-APDownload protocol
IP protocol for CPOX antibody 16724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols