CPSF4 Polyclonal antibody

• Featured Product
• KD/KO Validated

CPSF4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 15023-1-AP

Synonyms

Neb 1, NAR, CPSF30, CPSF 30 kDa subunit, Cleavage and polyadenylation specificity factor subunit 4

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Validation Data Gallery

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  WB analysis of HEK-293 using 15023-1-AP

  WB result of CPSF4 antibody (15023-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPSF4 transfected HEK-293 cells.

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  WB analysis of Jurkat using 15023-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15023-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15023-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15023-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15023-1-AP

  IP result of anti-CPSF4 (IP:15023-1-AP, 3ug; Detection:15023-1-AP 1:400) with HeLa cells lysate 1000ug.

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  IHC staining of human endometrial cancer using 15023-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15023-1-AP (CPSF4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human endometrial cancer using 15023-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15023-1-AP (CPSF4 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 15023-1-AP

  Immunofluorescent analysis of HepG2 cells, using CPSF4 antibody 15023-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells, HeLa cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

15023-1-AP targets CPSF4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPSF4 fusion protein Ag7103
• Full Name: cleavage and polyadenylation specific factor 4, 30kDa
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC003101
• Gene Symbol: CPSF4
• Gene ID (NCBI): 10898
• RRID: AB_2084544
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mature 3-prime ends of cellular mRNAs are producted by endonucleolytic cleavage of the primary transcripts, followed by polyadenylation of the upstream cleavage products. The cleavage and polyadenylation specificity factor (CPSF) has a essential role in pre-mRNA 3-prime end processing in that it is required in both the cleavage step and subsequent poly(A) addition [PMID: 9651582]. CPSF4 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, whose other components are CPSF1, CPSF2, CPSF3 and FIP1L1.

Publications

Species	Application	Title
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB,IP	Mol Oncol; CPSF4 activates telomerase reverse transcriptase and predicts poor prognosis in human lung adenocarcinomas.; Authors - Wangbing Chen ; KD Validated
human	WB,IHC,IF	Cancer Lett; Cleavage and polyadenylation specific factor 4 targets NF-κB/cyclooxygenase-2 signaling to promote lung cancer growth and progression.; Authors - Canhui Yi ; KD Validated
human	WB,IF	Mol Oncol; CREB-binding protein regulates lung cancer growth by targeting MAPK and CPSF4 signaling pathway.; Authors - Zhipeng Tang
pig	WB	Int J Biol Macromol; Key pituitary miRNAs mediate the expression of pig GHRHR splice variants by regulating splice factors.; Authors - Zhaohui Teng
human	WB, IF	Biochim Biophys Acta Mol Cell Res; Cleavage and polyadenylation specific factor 4 promotes colon cancer progression by transcriptionally activating hTERT.; Authors - Qian Yang