Product Information
Phalloidin is a toxin isolated from mushrooms that binds F-Actin in the cytoskeleton. This product is conjugated to our fluorescent CoraLite(R) dye to stain F-actin for labeling, identifying and quantifying actin filaments in immunostaining studies. Compared to many fluorescent dyes, CoraLite(R) dyes have advantages in brightness, light stability and water solubility. Excitation/Emission maxima wavelengths are 490 nm/515 nm.
Conjugated
CoraLite® Plus 488-Phalloidin (green)
Ex/Em: 490/515 nm
Storage
Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment.
Protocol
Stock preparation: Prepare a 200T/mL stock solution by dissolving lyophilized phalloidin (300T) in 1.5 mL sterile water and protect solution from light.
Cautions
This product is formulated as a freeze-dried powder. Please centrifuge it immediately before use and dissolve it in a suitable solvent before use. Phalloidin is highly toxic, please take protective measures while handling.
Note: Methanol can damage F-actin during fixation. It is best to avoid fixatives containing any methanol.
Stock preparation
Prepare a 200T/mL stock solution by dissolving lyophilized phalloidin (300T) in 1.5 mL sterile water and protect solution from light.
Cited in Article as
PF00001, CoraLite® Plus 488-Phalloidin (green), Proteintech, IL, USA
Publications
| Application | Title |
|---|---|
Science Hyaluronic acid and tissue mechanics orchestrate mammalian digit tip regeneration. | |
Cell RNA Pol II inhibition activates cell death independently from the loss of transcription | |
Cell Metab The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity | |
Mil Med Res D-mannose alleviates intervertebral disc degeneration through glutamine metabolism | |
Bioact Mater A novel mechanism of inhibiting in-stent restenosis with arsenic trioxide drug-eluting stent: Enhancing contractile phenotype of vascular smooth muscle cells via YAP pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kamal (Verified Customer) (10-16-2025) | The antibody was diluted in 1x PBS. Very nice staining. Worth it.
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FH Rushita (Verified Customer) (10-07-2025) | So easy to use and beautiful crisp images!
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FH Siavash (Verified Customer) (01-17-2025) | 4% PFA-fixed cells were permeabilized with 0.5% Triton X-100 in PBS prior to staining. Cells were incubated with CoraLite®488-conjugated Phalloidin for 20 min at room temperature on shaker. Picture is shown with maximum intensity projection. Vinculin (Red), Actin (Green), Nucleus (Blue).
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FH Udesh (Verified Customer) (10-04-2023) | Worked well at 1:300 for 1H in 2% BSA at Room Temperature
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FH Víctor (Verified Customer) (07-20-2023) | Really good antibody, at least in my staining of human fibroblasts. The intensity of the conjugated antibody is really good and it seems very specific binding microfilaments of the citoskeleton, with a very low background.
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FH Niklas (Verified Customer) (11-08-2022) | great staining with even a high dilution factor
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