CRTAM/CD355 Polyclonal antibody

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  WB analysis using 16627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16627-1-AP (CRTAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CRTAM/CD355 Polyclonal Antibody for WB, ELISA

Cat No. 16627-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

CRTAM, Cytotoxic and regulatory T-cell molecule, Class-I MHC-restricted T-cell-associated molecule, CD355

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse thymus tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16627-1-AP targets CRTAM/CD355 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRTAM/CD355 fusion protein Ag9956
• Full Name: cytotoxic and regulatory T cell molecule
• Calculated Molecular Weight: 393 aa, 45 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC070266
• Gene Symbol: CRTAM
• Gene ID (NCBI): 56253
• RRID: AB_3085502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95727
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223). The predicted molecular weight of CRTAM is 43 kDa. Due to glycosylation, the observed molecular weight of CRTAM can be apparent at ~80 kDa (PMID: 16300832).