CSGALNACT1 Polyclonal antibody

CSGALNACT1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26913-1-AP

Synonyms

beta4GalNAcT, Beta4GalNAcT 1, ChGn, CSGalNAcT 1, CSGALNACT1, GALNACT1

---

Validation Data Gallery

• 

  WB analysis of PC-3 using 26913-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26913-1-AP (CSGALNACT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 26913-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26913-1-AP (CSGALNACT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 26913-1-AP

  IP result of anti-CSGALNACT1 (IP:26913-1-AP, 4ug; Detection:26913-1-AP 1:1000) with PC-3 cells lysate 3200ug.

---

Tested Applications

• Positive WB detected in: PC-3 cells, MCF-7 cells
• Positive IP detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 26913-1-AP is CSGALNACT1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSGALNACT1 fusion protein Ag25455
• Full Name: chondroitin sulfate N-acetylgalactosaminyltransferase 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61-68 kDa
• GenBank Accession Number: BC060772
• Gene Symbol: CSGALNACT1
• Gene ID (NCBI): 55790
• RRID: AB_2880682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSGalNAcT-1 (chondroitin sulfate N-acetylgalactosaminyltransferase-1), catalyzing the transfer of a GalNAc residue onto the nonreducing end of GlcA, is a key glycosyltransferase for chondroitin sulfate (CS) biosynthesis in cartilage. Abnormal expression of CSGalNAcT-1 in cartilage has been linked to diseases like osteoarthritis.

Publications

Species	Application	Title
mouse	WB	Br J Pharmacol; Chondroitin sulfate proteoglycan is a potential target of memantine to improve cognitive function via the promotion of adult neurogenesis.; Authors - Shoichiro Maeda
	WB	Methods Mol Biol; Bacterial Amyloids.; Authors - Margery L Evans