CSN2 Polyclonal antibody

CSN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30498-1-AP

Synonyms

Beta casein, CASB, casein beta, CSN2

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Validation Data Gallery

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  WB analysis using 30498-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30498-1-AP (CSN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30498-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 30498-1-AP (CSN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast hyperplasia using 30498-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 30498-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 30498-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HT-29 cells, NIH/3T3 cells, mouse liver tissue, mouse testis tissue, DU 145 cells, rat liver tissue
• Positive IHC detected in: human breast hyperplasia tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30498-1-AP targets CSN2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSN2 fusion protein Ag27943
• Full Name: casein beta
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC096194
• Gene Symbol: CSN2
• Gene ID (NCBI): 1447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSN2 is also named as Beta-casein and CASB.The β-casein is the most abundant protein in camel milk, and is one of the most important milk protein fractions  (PMID: 24973699).