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  • KD/KO Validated

CSNK2B Polyclonal antibody

CSNK2B Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 20234-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

CSNK2B-specific, Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, A431 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20234-1-AP targets CSNK2B in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

fusion protein

Predict reactive species
Full Name casein kinase 2, beta polypeptide
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberNM_001320
Gene Symbol CSNK2B
Gene ID (NCBI) 1460
RRIDAB_10733246
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP67870
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK2B, also named as CK2N, G5A nd CK II beta, belongs to the casein kinase 2 subunit beta family. CSNK2B participates in Wnt signaling. It plays a complex role in regulating the basal catalytic activity of the alpha subunit. The antibody is specific to CSNK2B.

Protocols

Product Specific Protocols
WB protocol for CSNK2B antibody 20234-1-APDownload protocol
IHC protocol for CSNK2B antibody 20234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.

Authors - Ming Kong
mouseWB

Phytomedicine

Spiropachysine A suppresses hepatocellular carcinoma proliferation by inducing methuosis in vitro and in vivo.

Authors - Yuan Fang
humanWB,IF

Front Mol Neurosci

Lovastatin Alleviates α-Synuclein Aggregation and Phosphorylation in Cellular Models of Synucleinopathy.

Authors - Lijun Dai
humanIHC, WB

J Cell Commun Signal

CSNK2B contributes to colorectal cancer cell proliferation by activating the mTOR signaling.

Authors - Shijun Yu
  • KD Validated
mouseWB,IF

Exp Cell Res

Oocyte-specific deletion of Gsα induces oxidative stress and deteriorates oocyte quality in mice.

Authors - Yue Xie
  • KO Validated
humanWB

J Exp Clin Cancer Res

Overexpression of NELFE contributes to gastric cancer progression via Wnt/β-catenin signaling-mediated activation of CSNK2B expression.

Authors - Shijun Yu
  • KD Validated
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