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  • KD/KO Validated

CSNK2B-specific Polyclonal antibody

CSNK2B-specific Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20234-1-AP

Synonyms

Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, CSNK2B-specific, G5A, Phosvitin, Protein G5a



Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, A431 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20234-1-AP targets CSNK2B-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name casein kinase 2, beta polypeptide
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberNM_001320
Gene symbol CSNK2B
Gene ID (NCBI) 1460
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSNK2B, also named as CK2N, G5A nd CK II beta, belongs to the casein kinase 2 subunit beta family. CSNK2B participates in Wnt signaling. It plays a complex role in regulating the basal catalytic activity of the alpha subunit. The antibody is specific to CSNK2B.

Protocols

Product Specific Protocols
WB protocol for CSNK2B-specific antibody 20234-1-APDownload protocol
IHC protocol for CSNK2B-specific antibody 20234-1-APDownload protocol
FC protocol for CSNK2B-specific antibody 20234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Mol Cell

Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities.

Authors - Andrea Scelfo
humanWB

J Exp Clin Cancer Res

Overexpression of NELFE contributes to gastric cancer progression via Wnt/β-catenin signaling-mediated activation of CSNK2B expression.

Authors - Shijun Yu
  • KD Validated
mouseWB,IF

Exp Cell Res

Oocyte-specific deletion of Gsα induces oxidative stress and deteriorates oocyte quality in mice.

Authors - Yue Xie
  • KO Validated
humanIHC, WB

J Cell Commun Signal

CSNK2B contributes to colorectal cancer cell proliferation by activating the mTOR signaling.

Authors - Shijun Yu
  • KD Validated
humanWB,IF

Front Mol Neurosci

Lovastatin Alleviates α-Synuclein Aggregation and Phosphorylation in Cellular Models of Synucleinopathy.

Authors - Lijun Dai