Tested Applications
| Positive WB detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55027-1-AP targets CSPG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | chondroitin sulfate proteoglycan 4 |
| Calculated Molecular Weight | 251 kDa |
| Observed Molecular Weight | 240-250 kDa, 450kDa |
| GenBank Accession Number | NM_001897 |
| Gene Symbol | CSPG4 |
| Gene ID (NCBI) | 1464 |
| RRID | AB_10791888 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UVK1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSPG4, also named as HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16 and NG2, is a proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. CSPG4 may inhibit neurite outgrowth and growth cone collapse during axon regeneration. It is cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. CSPG4 may regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. It modulates the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. CSPG4 functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. It promotes retraction fiber formation and cell polarization through Rho GTPase activation and stimulates alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. CSPG4 activates FAK and ERK1/ERK2 signaling cascades. The antibody is specific to CSPG4.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CSPG4 antibody 55027-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CSPG4/NG2 Polyclonal antibody (Cat# 55027-1-AP) has 49 citations across journals including Nature Genetics, Nature Cell Biology, Nature Communications, Biomaterials, Journal of Clinical Investigation, and Theranostics. Research fields span neuroregeneration and spinal cord injury, cancer biology and metastasis, pericyte/vascular biology, fibrosis, neuroinflammation, tissue engineering, and diabetes. Applications cited include WB and IHC in human, mouse, and rat models.
| Species | Application | Title |
|---|---|---|
Nat Genet Single-cell multi-omic and spatial profiling of esophageal squamous cell carcinoma reveals the immunosuppressive role of GPR116+ pericytes in cancer metastasis | ||
Nat Cell Biol A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. | ||
Nat Commun Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection | ||
Nat Commun Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system | ||
Nat Commun FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment | ||
Gut Microbes Maternal-infant probiotic transmission mitigates early-life stress-induced autism in mice |
Reviews
What customers say
All three reviews are negative. One user reported a weak band at the expected molecular weight in Western Blot but noted many strong nonspecific bands. Two other users tested the antibody by IHC on human tissue (lung, skin, and brain cortex) and both reported high background with no specific staining, even after trying different antigen retrieval conditions (pH6 citrate and pH9). Overall, users found the antibody to have poor specificity and high background across applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
X (Verified Customer) (07-11-2022) | Although there is a weak band at the right MW, there are many other strong nonspecific bands.
|
Kinan (Verified Customer) (04-22-2022) | No specific staining on human lung and skin sections. Background signal was very high also. HIER pH6 in citrate buffer.
|
Nadine (Verified Customer) (01-31-2019) | We had high background and no specific staining of IHC-paraffin slides of human brain cortex. We tried both pH6 and pH9 antigen retrieval solutions. We have not tested other applications.
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