Tested Applications
Positive WB detected in | HepG2 cells, mouse kidney tissue, rat kidney tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 4 publications below |
WB | See 7 publications below |
IHC | See 4 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
CoIP | See 2 publications below |
Product Information
10892-2-AP targets CSRP2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CSRP2 fusion protein Ag1338 Predict reactive species |
Full Name | cysteine and glycine-rich protein 2 |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 21 kDa |
GenBank Accession Number | BC000992 |
Gene Symbol | CSRP2 |
Gene ID (NCBI) | 1466 |
RRID | AB_2087738 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16527 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSRP2 (Cysteine and glycine-rich protein 2) is a member of the LIM-only family of cysteine-rich proteins and contains two LIM domains, which has been recently implicated in the progression and metastasis of a variety of cancers. CSRP2 is functionally linked to the activity of TGF-b1 in hepatic stellate cells (HSC), derivatives thereof, and in differentiated VSMC.CSRP2 knockdown signifcantly inhibits hypoxia-stimulated invadopodium formation, ECM degradation and invasion in MDA-MB-231 cells (PMID: 33042270). CSRP2 is a short (21 kDa) two LIM domain-containing protein, which is upregulated in invasive breast cancer cells, and localizes along the protrusive actin core of invadopodium1 (PMID: 29976963, 16735029).
Protocols
Product Specific Protocols | |
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WB protocol for CSRP2 antibody 10892-2-AP | Download protocol |
IHC protocol for CSRP2 antibody 10892-2-AP | Download protocol |
IP protocol for CSRP2 antibody 10892-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Rep Proteotoxicity in cardiac amyloidosis: amyloidogenic light chains affect the levels of intracellular proteins in human heart cells. | ||
Toxicol Appl Pharmacol Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif
| ||
Cell Biochem Funct Cysteine and glycine-rich protein 2 retards platelet-derived growth factor-BB-evoked phenotypic transition of airway smooth muscle cells by decreasing YAP/TAZ activity
| ||
Toxicol Appl Pharmacol Cysteine and glycine-rich protein 2 is crucial for maintaining the malignant phenotypes of gliomas through its action on Notch signalling cascade | ||
Oncol Rep Cysteine‑ and glycine‑rich protein 2: A vital regulator that inhibits necroptosis glioma cell by activating the JAK‑STAT1 pathways
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