CSRP2 Polyclonal antibody

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  WB analysis using 10892-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10892-2-AP (CSRP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10892-2-AP

  IP result of anti-CSRP2 (IP:10892-2-AP, 4ug; Detection:10892-2-AP 1:1000) with mouse liver tissue lysate 4000ug.

• Featured Product
• KD/KO Validated

CSRP2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 10892-2-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

CRP2, CSRP2, Cysteine rich protein 2, LIM domain only protein 5, LMO 5, LMO5, SmLIM, Smooth muscle cell LIM protein

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse kidney tissue, rat kidney tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

10892-2-AP targets CSRP2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSRP2 fusion protein Ag1338
• Full Name: cysteine and glycine-rich protein 2
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC000992
• Gene Symbol: CSRP2
• Gene ID (NCBI): 1466
• RRID: AB_2087738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16527
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Rep; Proteotoxicity in cardiac amyloidosis: amyloidogenic light chains affect the levels of intracellular proteins in human heart cells.; Authors - Esther Imperlini
rat	WB,IF	Toxicol Appl Pharmacol; Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif; Authors - Xinghe Chen ; KD Validated
human	WB,IHC	J Med Dent Sci; TDP-43 in the skin of amyotrophic lateral sclerosis patients.; Authors - Keisuke Abe
mouse	WB	Cell Biochem Funct; Cysteine and glycine-rich protein 2 retards platelet-derived growth factor-BB-evoked phenotypic transition of airway smooth muscle cells by decreasing YAP/TAZ activity; Authors - Huiyuan Wang ; KD Validated
human,mouse	WB,IHC	Toxicol Appl Pharmacol; Cysteine and glycine-rich protein 2 is crucial for maintaining the malignant phenotypes of gliomas through its action on Notch signalling cascade; Authors - Lingtong Liu
human	WB,IHC,CoIP	Oncol Rep; Cysteine‑ and glycine‑rich protein 2: A vital regulator that inhibits necroptosis glioma cell by activating the JAK‑STAT1 pathways; Authors - Dongsheng Lv ; KD Validated