CST4 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of human saliva using 86955-1-RR (same clone as 86955-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 86955-1-RR (CST4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86955-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86955-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86955-1-RR against Human CST4 were performed. The affinity constant is 0.257 nM.

CST4 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86955-1-PBS

Clone No.252073B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Cystatin 4, cystatin S, Cystatin-4, Cystatin-S, Cystatin-SA-III

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Product Information

86955-1-PBS targets CST4 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3098

  Product name: recombinant human CST4 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 21-141 aa of NM_001899.2

  Sequence: SSSKEENRIIPGGIYDADLNDEWVQRALHFAISEYNKATEDEYYRRPLQVLRAREQTFGGVNYFFDVEVGRTICTKSQPNLDTCAFHEQPELQKKQLCSFEIYEVPWEDRMSLVNSRCQEA

• Full Name: cystatin S
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: NM_001899.2
• Gene Symbol: Cystatin S
• Gene ID (NCBI): 1472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01036
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cystatins are a family of inhibitors of cysteine peptidases that comprises the salivary cystatins D and S-type (including CST5 and CST4,1,2) and cystatin C-type (CST3) (PMID: 25329717). The D and S-type cystatin have also been detected in seminal plasma, tears, and tracheobronchial fluid but not in other fluids and secretions where cystatin C is found. And the CST4 was expressed in the serous acinar and demilune cells of the human submandibular gland and at lower levels in the serous acini of the parotid gland (PMID:11879580). CST4 specifically combines with cysteine protease to regulate its activity, thus preventing hydrolysis of the extracellular matrix. And some studies propose that CST4 might be a biomarker for gastrointestinal cancer (PMID:29218251; PMID:29636621).