CSTF1 Polyclonal antibody, PBS Only

• 

  WB analysis using 10064-2-AP (same clone as 10064-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10064-2-AP (CSTF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10064-2-PBS in a different storage buffer formulation.

• 

  IP experiment of NIH/3T3 using 10064-2-AP (same clone as 10064-2-PBS)

  IP result of anti-CSTF1 (IP:10064-2-AP, 2ug; Detection:10064-2-AP 1:500) with NIH/3T3 cells lysate 2500ug. This data was developed using the same antibody clone with 10064-2-PBS in a different storage buffer formulation.

CSTF1 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 10064-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

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Product Information

10064-2-PBS targets CSTF1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0109

  Product name: Recombinant human CSTF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 143-419 aa of BC001011

  Sequence: KSAMPIEVMMNETAQQNMENHPVIRTLYDHVDEVTCLAFHPTEQILASGSRDYTLKLFDYSKPSAKRAFKYIQEAEMLRSISFHPSGDFILVGTQHPTLRLYDINTFQCFVSCNPQDQHTDAICSVNYNSSANMYVTGSKDGCIKLWDGVSNRCITTFEKAHDGAEVCSAIFSKNSKYILSSGKDSVAKLWEISTGRTLVRYTGAGLSGRQVHRTQAVFNHTEDYVLLPDERTISLCCWDSRTAERRNLLSLGHNNIVRCIVHSPTNPGFMTCSDDF

• Full Name: cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
• Calculated Molecular Weight: 48.3 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC001011
• Gene Symbol: CSTF1
• Gene ID (NCBI): 1477
• RRID: AB_2261145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05048
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 subunit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD). This subunit may be responsible for the interaction of CSTF with other factors to form a satable complex on the pre-mRNA, and multiple WD domains are required for protein interactions.