CTDSP2 Polyclonal antibody

CTDSP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11116-1-AP

Synonyms

CTDSP2, NIF2, NLI interacting factor 2, OS4, Protein OS 4, PSR2, SCP2, Small CTD phosphatase 2

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Validation Data Gallery

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  WB analysis of human kidney using 11116-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11116-1-AP (CTDSP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11116-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11116-1-AP (CTDSP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11116-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11116-1-AP (CTDSP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11116-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11116-1-AP (CTDSP2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human kidney tissue, HepG2 cells, K-562 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11116-1-AP targets CTDSP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTDSP2 fusion protein Ag1601
• Full Name: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC065920
• Gene Symbol: CTDSP2
• Gene ID (NCBI): 10106
• RRID: AB_2087086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTDSPs are C-class phosphatases that act on Ser 5 of the Pol II C-terminal domain (CTD) to decrease transcription [PMID:15681389]. CTDSP enzymatic activity is required for REST function, and knockdown of CTDSP induces the neuronal differentiation of mouse embryonic carcinoma P19 cells. CTDSP2 has been shown to enhance TGF-β signaling by the dephosphorylation of SMAD proteins and attenuates androgen receptor-mediated transcription [PMID:16724108]. It negatively regulates RNA polymerase II transcription by controlling the transition from initiation/capping to processive transcript elongation. It was recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells[PMID: 12721286].