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  • KD/KO Validated

CUL2 Polyclonal antibody

CUL2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10981-2-AP

Synonyms

CUL 2, CUL2, cullin 2



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10981-2-AP targets CUL2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL2 fusion protein Ag1434
Full Name cullin 2
Calculated molecular weight 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC009591
Gene symbol CUL2
Gene ID (NCBI) 8453
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for CUL2 antibody 10981-2-APDownload protocol
IHC protocol for CUL2 antibody 10981-2-APDownload protocol
IF protocol for CUL2 antibody 10981-2-APDownload protocol
IP protocol for CUL2 antibody 10981-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int Immunopharmacol

Aberrant expression of microRNAs in gastric cancer and biological significance of miR-574-3p.

Authors - Su Yingying Y
humanWB

Cell Physiol Biochem

A Human Long Non-Coding RNA ALT1 Controls the Cell Cycle of Vascular Endothelial Cells Via ACE2 and Cyclin D1 Pathway.

Authors - Wen Li
humanWB

J Cell Sci

Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.

Authors - Tomoaki Nagai
  • KD Validated
humanWB

Mol Cell Biol

DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.

Authors - Chen Hao H
  • KD Validated
humanWB

Dev Cell

AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.

Authors - Manuela Antonioli
  • KD Validated