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CUL4A Monoclonal antibody

CUL4A Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, monkey, mouse, rat, pig







Cat no : 66038-1-Ig


CUL 4A, CUL4A, cullin 4A

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, pig brain tissue, MCF-7 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman heart tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66038-1-Ig targets CUL4A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, monkey, mouse, rat, pig samples.

Tested Reactivity human, monkey, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CUL4A fusion protein Ag18089
Full Name cullin 4A
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 77 kDa, 88 kDa
GenBank Accession NumberBC008308
Gene Symbol CUL4A
Gene ID (NCBI) 8451
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.


Product Specific Protocols
WB protocol for CUL4A antibody 66038-1-IgDownload protocol
IHC protocol for CUL4A antibody 66038-1-IgDownload protocol
IF protocol for CUL4A antibody 66038-1-IgDownload protocol
IP protocol for CUL4A antibody 66038-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Curr Biol

Temporal Regulation of ESCO2 Degradation by the MCM Complex, the CUL4-DDB1-VPRBP Complex, and the Anaphase-Promoting Complex.

Authors - Masashi Minamino


GSK3β-driven SOX2 overexpression is a targetable vulnerability in esophageal squamous cell carcinoma

Authors - Li Kang
  • KD Validated

Stem Cell Res Ther

KAP1 phosphorylation promotes the survival of neural stem cells after ischemia/reperfusion by maintaining the stability of PCNA.

Authors - Wan Wang

Cell Death Differ

AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner

Authors - Weimin Xu